Transcriptional regulation of central carbon metabolism in Pseudomonas aeruginosa.
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Dolan, S., Pereira, G., Silva-Rocha, R., & Welch, M. (2020). Transcriptional regulation of central carbon metabolism in Pseudomonas aeruginosa.. Microbial biotechnology, 13 (1), 285-289. https://doi.org/10.1111/1751-7915.13423
Microbes such as Pseudomonas aeruginosa are often challenged by rapidly changing nutritional environments. In order to adapt to these shifts in nutrient availability, bacteria exert tight transcriptional control over the enzymes of central metabolism. This transcriptional control is orchestrated by a series of transcriptional repressors and activators. Although a number of these transcription factors have been identified, many others remain uncharacterised. Here, we present a simple pipeline to uncover and validate the targets of uncharacterised transcriptional regulators in P. aeruginosa. We use this approach to identify and confirm that an orthologue of the Pseudomonas fluorescens transcriptional regulator (RccR) binds to the upstream region of isocitrate lyase (aceA) in P. aeruginosa, thereby repressing flux through the glyoxylate shunt during growth on non-C2 carbon sources.
Pseudomonas aeruginosa, Carbon, Isocitrate Lyase, Bacterial Proteins, Gene Expression Regulation, Bacterial
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External DOI: https://doi.org/10.1111/1751-7915.13423
This record's URL: https://www.repository.cam.ac.uk/handle/1810/292274
Attribution 4.0 International
Licence URL: https://creativecommons.org/licenses/by/4.0/