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dc.contributor.authorLiu, Zheng Jen
dc.contributor.authorMartínez Cuesta, Sergioen
dc.contributor.authorvan Delft, Pieteren
dc.contributor.authorBalasubramanian, Shankaren
dc.date.accessioned2019-05-03T23:32:29Z
dc.date.available2019-05-03T23:32:29Z
dc.date.issued2019-07en
dc.identifier.issn1755-4330
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/292408
dc.description.abstractIn DNA, the loss of a nucleobase by hydrolysis generates an abasic site. Formed as a result of DNA damage, as well as a key intermediate during the base excision repair pathway, abasic sites are frequent DNA lesions which can lead to mutations and strand breaks. Here we present snAP-seq, a chemical approach that selectively exploits the reactive aldehyde moiety at abasic sites to reveal their location within DNA at single-nucleotide resolution. Importantly, the approach resolves abasic sites from other aldehyde functionalities known to exist in genomic DNA. snAP-seq was validated on synthetic DNA, then applied to two separate genomes. We studied the distribution of thymine modifications in the Leishmania major genome by enzymatically converting these modifications into abasic sites followed by abasic site mapping. We also apply snAP-seq directly to HeLa DNA to provide a map of endogenous abasic sites in human genomic DNA.
dc.description.sponsorshipWellcome Trust (grant no. 209441/Z/17/Z) Pembroke College, Cambridge and the Herchel Smith Fund Marie Curie Fellow of the European Union (grant no. FP7-PEOPLE-2013-IEF/624885)
dc.format.mediumPrint-Electronicen
dc.languageengen
dc.publisherNature Publishing Group
dc.rightsAll rights reserved
dc.subjectHela Cellsen
dc.subjectHumansen
dc.subjectLeishmania majoren
dc.subjectDNA Damageen
dc.subjectAldehydesen
dc.subjectThymineen
dc.subjectDNA-(Apurinic or Apyrimidinic Site) Lyaseen
dc.subjectDNAen
dc.subjectMolecular Probesen
dc.subjectSequence Analysis, DNAen
dc.subjectBase Sequenceen
dc.subjectGenomeen
dc.subjectUracil-DNA Glycosidaseen
dc.subjectGene Knockdown Techniquesen
dc.titleSequencing abasic sites in DNA at single-nucleotide resolution.en
dc.typeArticle
prism.endingPage637
prism.issueIdentifier7en
prism.publicationDate2019en
prism.publicationNameNature chemistryen
prism.startingPage629
prism.volume11en
dc.identifier.doi10.17863/CAM.39558
dcterms.dateAccepted2019-04-30en
rioxxterms.versionofrecord10.1038/s41557-019-0279-9en
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2019-07en
dc.contributor.orcidMartínez Cuesta, Sergio [0000-0001-9806-2805]
dc.contributor.orcidBalasubramanian, Shankar [0000-0002-0281-5815]
dc.identifier.eissn1755-4349
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idWellcome Trust (099232/Z/12/Z)
pubs.funder-project-idWellcome Trust (209441/Z/17/Z)
cam.orpheus.successThu Jan 30 10:47:47 GMT 2020 - Embargo updated*
rioxxterms.freetoread.startdate2020-01-31


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