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dc.contributor.authorWrzaczek, Michael
dc.contributor.authorVainonen, Julia P
dc.contributor.authorStael, Simon
dc.contributor.authorTsiatsiani, Liana
dc.contributor.authorHelp-Rinta-Rahko, Hanna
dc.contributor.authorGauthier, Adrien
dc.contributor.authorKaufholdt, David
dc.contributor.authorBollhöner, Benjamin
dc.contributor.authorLamminmäki, Airi
dc.contributor.authorStaes, An
dc.contributor.authorGevaert, Kris
dc.contributor.authorTuominen, Hannele
dc.contributor.authorVan Breusegem, Frank
dc.contributor.authorHelariutta, Ykä
dc.contributor.authorKangasjärvi, Jaakko
dc.date.accessioned2019-05-08T14:25:39Z
dc.date.available2019-05-08T14:25:39Z
dc.date.issued2015-01-02
dc.identifier.issn0261-4189
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/292544
dc.description.abstractRecognition of extracellular peptides by plasma membrane-localized receptor proteins is commonly used in signal transduction. In plants, very little is known about how extracellular peptides are processed and activated in order to allow recognition by receptors. Here, we show that induction of cell death in planta by a secreted plant protein GRIM REAPER (GRI) is dependent on the activity of the type II metacaspase METACASPASE-9. GRI is cleaved by METACASPASE-9 in vitro resulting in the release of an 11 amino acid peptide. This peptide bound in vivo to the extracellular domain of the plasma membrane-localized, atypical leucine-rich repeat receptor-like kinase POLLEN-SPECIFIC RECEPTOR-LIKE KINASE 5 (PRK5) and was sufficient to induce oxidative stress/ROS-dependent cell death. This shows a signaling pathway in plants from processing and activation of an extracellular protein to recognition by its receptor.
dc.format.mediumPrint-Electronic
dc.languageeng
dc.publisherEMBO
dc.subjectCell Membrane
dc.subjectArabidopsis
dc.subjectCaspases
dc.subjectProtein Kinases
dc.subjectPeptides
dc.subjectArabidopsis Proteins
dc.subjectSignal Transduction
dc.subjectCell Death
dc.subjectProtein Structure, Tertiary
dc.subjectProtein Binding
dc.subjectOxidative Stress
dc.titleGRIM REAPER peptide binds to receptor kinase PRK5 to trigger cell death in Arabidopsis.
dc.typeArticle
prism.endingPage66
prism.issueIdentifier1
prism.publicationDate2015
prism.publicationNameEMBO J
prism.startingPage55
prism.volume34
dc.identifier.doi10.17863/CAM.39704
rioxxterms.versionofrecord10.15252/embj.201488582
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2015-01
dc.contributor.orcidHelariutta, Yrjo [0000-0002-7287-8459]
dc.identifier.eissn1460-2075
rioxxterms.typeJournal Article/Review
pubs.funder-project-idEuropean Research Council (323052)
pubs.funder-project-idGatsby Charitable Foundation (GAT3395/PR3)
cam.issuedOnline2014-11-14


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