Magnetic Resonance Imaging Is More Sensitive Than PET for Detecting Treatment-Induced Cell Death-Dependent Changes in Glycolysis.
Hesketh, Richard L
American Association for Cancer Research (AACR)
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Hesketh, R. L., Wang, J., Wright, A., Lewis, D. Y., Denton, A. E., Grenfell, R., Miller, J., et al. (2019). Magnetic Resonance Imaging Is More Sensitive Than PET for Detecting Treatment-Induced Cell Death-Dependent Changes in Glycolysis.. Cancer research, 79 (14), 3557-3569. https://doi.org/10.1158/0008-5472.can-19-0182
Metabolic imaging has been widely used to measure the early responses of tumors to treatment. Here we assess the abilities of positron emission tomography (PET) measurement of [18F]FDG uptake and magnetic resonance imaging (MRI) measurement of hyperpolarized [1-13C]pyruvate metabolism to detect early changes in glycolysis following treatment-induced cell death in human colorectal (Colo205) and breast adenocarcinoma (MDA-MB-231) xenografts in mice. A TRAIL agonist that binds to human but not mouse cells induced tumor-selective cell death. Tumor glycolysis was assessed by injecting [1,6-13C2]glucose and measuring 13C-labeled metabolites in tumor extracts. Injection of hyperpolarized [1-13C]pyruvate induced rapid reduction in lactate labeling. This decrease, which correlated with an increase in histological markers of cell death and preceded decrease in tumor volume, reflected reduced flux from glucose to lactate and decreased lactate concentration. However, [18F]FDG uptake and phosphorylation was maintained following treatment, which has been attributed previously to increased [18F]FDG uptake by infiltrating immune cells. Quantification of [18F]FDG uptake in flow-sorted tumor and immune cells from disaggregated tumors identified CD11b+/CD45+ macrophages as the most [18F]FDG-avid cell type present, yet they represented <5% of the cells present in the tumors and could not explain the failure of [18F]FDG-PET to detect treatment response. MRI measurement of hyperpolarized [1-13C]pyruvate metabolism is therefore a more sensitive marker of the early decreases in glycolytic flux that occur following cell death than PET measurements of [18F]FDG uptake.
Cell Line, Tumor, Animals, Mice, Inbred BALB C, Humans, Mice, Nude, Adenocarcinoma, Colorectal Neoplasms, Carbon Isotopes, Lactic Acid, Pyruvic Acid, Fluorodeoxyglucose F18, Antineoplastic Agents, Radiopharmaceuticals, Positron-Emission Tomography, Magnetic Resonance Imaging, Cell Death, Glycolysis, Female, Receptors, TNF-Related Apoptosis-Inducing Ligand, Heterografts, Triple Negative Breast Neoplasms
Cancer Research UK (16465)
Cancer Research UK (C14303_do not transfer)
Aarhus University (Source: Data Science Research Centre (DSRC)) (15952)
Cancer Research UK (25040)
Rosetrees Trust (A1698)
Rosetrees Trust (M723)
External DOI: https://doi.org/10.1158/0008-5472.can-19-0182
This record's URL: https://www.repository.cam.ac.uk/handle/1810/292968
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