Direct Depolymerization Coupled to Liquid Extraction Surface Analysis-High-Resolution Mass Spectrometry for the Characterization of the Surface of Plant Tissues.
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Publication Date
2019-07-02Journal Title
Anal Chem
ISSN
0003-2700
Publisher
American Chemical Society (ACS)
Volume
91
Issue
13
Pages
8326-8333
Language
eng
Type
Article
This Version
AM
Physical Medium
Print-Electronic
Metadata
Show full item recordCitation
Giorio, C., Moyroud, E., Glover, B., & Kalberer, M. (2019). Direct Depolymerization Coupled to Liquid Extraction Surface Analysis-High-Resolution Mass Spectrometry for the Characterization of the Surface of Plant Tissues.. Anal Chem, 91 (13), 8326-8333. https://doi.org/10.1021/acs.analchem.9b01094
Abstract
The cuticle, the outermost layer covering the epidermis of most aerial organs of land plants, can have a heterogeneous composition even on the surface of the same organ. The main cuticle component is the polymer cutin which, depending on its chemical composition and structure, can have different biophysical properties. In this study, we introduce a new on-surface depolymerization method coupled to liquid extraction surface analysis (LESA) high-resolution mass spectrometry (HRMS) for a fast and spatially resolved chemical characterization of the cuticle of plant tissues. The method is composed of an on-surface saponification, followed by extraction with LESA using a chloroform-acetonitrile-water (49:49:2) mixture and direct HRMS detection. The method is also compared with LESA-HRMS without prior depolymerization for the analysis of the surface of the petals of Hibiscus richardsonii flowers, which have a ridged cuticle in the proximal region and a smooth cuticle in the distal region. We found that on-surface saponification is effective enough to depolymerize the cutin into its monomeric constituents thus allowing detection of compounds that were not otherwise accessible without a depolymerization step. The effect of the depolymerization procedure was more pronounced for the ridged/proximal cuticle, which is thicker and richer in epicuticular waxes compared with the cuticle in the smooth/distal region of the petal.
Sponsorship
European Research Council (ERC consolidator grant 279405)
the Herchel Smith fund
the Gatsby Charitable Foundation
BBSRC grant BB/P001157/1
Funder references
Gatsby Charitable Foundation (GAT3395/GLG)
Biotechnology and Biological Sciences Research Council (BB/P001157/1)
European Research Council (279405)
European Commission (301472)
Identifiers
External DOI: https://doi.org/10.1021/acs.analchem.9b01094
This record's URL: https://www.repository.cam.ac.uk/handle/1810/293239
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