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dc.contributor.authorGiorio, Chiara
dc.contributor.authorMoyroud, Edwige
dc.contributor.authorGlover, Beverley J
dc.contributor.authorKalberer, Markus
dc.date.accessioned2019-05-29T23:30:43Z
dc.date.available2019-05-29T23:30:43Z
dc.date.issued2019-07-02
dc.identifier.issn0003-2700
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/293239
dc.description.abstractThe cuticle, the outermost layer covering the epidermis of most aerial organs of land plants, can have a heterogeneous composition even on the surface of the same organ. The main cuticle component is the polymer cutin which, depending on its chemical composition and structure, can have different biophysical properties. In this study, we introduce a new on-surface depolymerization method coupled to liquid extraction surface analysis (LESA) high-resolution mass spectrometry (HRMS) for a fast and spatially resolved chemical characterization of the cuticle of plant tissues. The method is composed of an on-surface saponification, followed by extraction with LESA using a chloroform-acetonitrile-water (49:49:2) mixture and direct HRMS detection. The method is also compared with LESA-HRMS without prior depolymerization for the analysis of the surface of the petals of Hibiscus richardsonii flowers, which have a ridged cuticle in the proximal region and a smooth cuticle in the distal region. We found that on-surface saponification is effective enough to depolymerize the cutin into its monomeric constituents thus allowing detection of compounds that were not otherwise accessible without a depolymerization step. The effect of the depolymerization procedure was more pronounced for the ridged/proximal cuticle, which is thicker and richer in epicuticular waxes compared with the cuticle in the smooth/distal region of the petal.
dc.description.sponsorshipEuropean Research Council (ERC consolidator grant 279405) the Herchel Smith fund the Gatsby Charitable Foundation BBSRC grant BB/P001157/1
dc.format.mediumPrint-Electronic
dc.languageeng
dc.publisherAmerican Chemical Society (ACS)
dc.rightsAll rights reserved
dc.titleDirect Depolymerization Coupled to Liquid Extraction Surface Analysis-High-Resolution Mass Spectrometry for the Characterization of the Surface of Plant Tissues.
dc.typeArticle
prism.endingPage8333
prism.issueIdentifier13
prism.publicationDate2019
prism.publicationNameAnal Chem
prism.startingPage8326
prism.volume91
dc.identifier.doi10.17863/CAM.40389
dcterms.dateAccepted2019-05-24
rioxxterms.versionofrecord10.1021/acs.analchem.9b01094
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2019-07
dc.contributor.orcidGiorio, Chiara [0000-0001-7821-7398]
dc.identifier.eissn1520-6882
rioxxterms.typeJournal Article/Review
pubs.funder-project-idGatsby Charitable Foundation (GAT3395/GLG)
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/P001157/1)
pubs.funder-project-idEuropean Research Council (279405)
pubs.funder-project-idEuropean Commission (301472)
cam.issuedOnline2019-05-24
cam.orpheus.successTue Jun 16 10:42:03 BST 2020 - Embargo updated
cam.orpheus.counter12
rioxxterms.freetoread.startdate2020-07-31


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