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dc.contributor.authorLilley, Kathrynen
dc.contributor.authorParsons, Harrieten
dc.date.accessioned2019-06-12T23:30:11Z
dc.date.available2019-06-12T23:30:11Z
dc.identifier.issn1040-4651
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/293579
dc.description.abstractThe order of enzymatic activity across Golgi cisternae is essential for complex molecule biosynthesis. However, an inability to separate Golgi cisternae has meant the cisternal distribution of most resident proteins, and their underlying localization mechanisms, are unknown. Here, we exploit differences in surface charge of intact cisternae to perform the first separation of early to late Golgi sub-compartments. We determine protein and glycan abundance profiles across the Golgi; over 390 resident proteins are identified, including 136 new additions, with over 180 cisternal assignments. These assignments provide a means to better understand the functional roles of Golgi proteins and how they operate sequentially. Protein and glycan distributions are validated in-vivo, using high resolution microscopy. Results reveal distinct functional compartmentalization among resident Golgi proteins. Analysis of transmembrane proteins shows several sequence-based characteristics relating to pI, hydrophobicity, Ser abundance and Phe bilayer asymmetry that change across the Golgi. Overall this suggests a continuum of TM features, rather than discrete rules, which guide proteins to earlier or later locations within the Golgi stack.
dc.description.sponsorshipMarie Curie fellowship PIEF-GA-2011-301401 1058 (FP7-PEOPLE-2011) MRC
dc.publisherAmerican Society of Plant Biologists
dc.rightsAll rights reserved
dc.rights.uri
dc.titleSeparating Golgi proteins from cis to trans reveals underlying properties of cisternal localizationen
dc.typeArticle
prism.endingPage2034
prism.issueIdentifier9en
prism.publicationNameThe Plant Cellen
prism.startingPage2010
prism.volume31en
dc.identifier.doi10.17863/CAM.40713
dcterms.dateAccepted2019-06-04en
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2019-06-04en
dc.contributor.orcidLilley, Kathryn [0000-0003-0594-6543]
dc.identifier.eissn1532-298X
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idWELLCOME TRUST (108467/Z/15/Z)
cam.issuedOnline2019-09-04en


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