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A MILI-independent piRNA biogenesis pathway empowers partial germline reprogramming.

Accepted version
Peer-reviewed

Type

Article

Change log

Authors

Vasiliauskaitė, Lina  ORCID logo  https://orcid.org/0000-0003-0926-3862
Berrens, Rebecca V 
Carrieri, Claudia 
Reik, Wolf 

Abstract

In mice, the pathway involving PIWI and PIWI-interacting RNA (PIWI-piRNA) is essential to re-establish transposon silencing during male-germline reprogramming. The cytoplasmic PIWI protein MILI mediates piRNA-guided transposon RNA cleavage as well as piRNA amplification. MIWI2's binding to piRNA and its nuclear localization are proposed to be dependent upon MILI function. Here, we demonstrate the existence of a piRNA biogenesis pathway that sustains partial MIWI2 function and reprogramming activity in the absence of MILI.

Description

Keywords

Animals, Argonaute Proteins, Gene Expression Regulation, Mice, Mice, Knockout, RNA, Small Interfering

Journal Title

Nat Struct Mol Biol

Conference Name

Journal ISSN

1545-9993
1545-9985

Volume Title

24

Publisher

Springer Science and Business Media LLC

Rights

All rights reserved