Overexpression of Osmosensitive Ca2+-Permeable Channel TMEM63B Promotes Migration in HEK293T Cells.
Marques, Marta C
Albuquerque, Inês S
Lopes Bernardes, Goncalo
American Chemical Society (ACS)
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Marques, M. C., Albuquerque, I. S., Vaz, S. H., & Lopes Bernardes, G. (2019). Overexpression of Osmosensitive Ca2+-Permeable Channel TMEM63B Promotes Migration in HEK293T Cells.. Biochemistry, 58 (26), 2861-2866. https://doi.org/10.1021/acs.biochem.9b00224
The recent discovery of the osmosensitive calcium (Ca2+) channel OSCA has revealed the potential mechanism by which plant cells sense diverse stimuli. Osmosensory transporters and mechanosensitive channels can detect and respond to osmotic shifts that play an important role in active cell homeostasis. Members of the TMEM63 family of proteins are described as the closest homologues of OSCAs. Here, we characterize TMEM63B, a mammalian homologue of OSCAs, recently classified as mechanosensitive. In HEK293T cells, TMEM63B localizes to the plasma membrane and is associated with F-actin. This Ca2+-permeable channel specifically induces Ca2+ influx across the membrane in response to extracellular Ca2+ concentration and hyperosmolarity. In addition, overexpression of TMEM63B in HEK293T cells significantly enhanced cell migration and wound healing. The link between Ca2+ osmosensitivity and cell migration might help to establish TMEM63B’s pathogenesis, for example, in cancer in which it is frequently overexpressed.
Cell Membrane, Epithelial Cells, Humans, Calcium, Actins, Calcium Channels, Wound Healing, Cell Movement, Up-Regulation, Models, Molecular, HEK293 Cells
Funded by the Royal Society (URF\R\180019 to G.J.L.B.) and FCT Portugal (iFCT IF/00624/2015 to G.J.L.B., Postdoctoral Fellowships SFRH/BPD/118731/2016 to M.C.M. and SFRH/BPD/81627/2011 to S.H.V., and Doctoral Studentship SFRH/BD/111556/2015 to I.S.A.).
Royal Society (URF\R\180019)
External DOI: https://doi.org/10.1021/acs.biochem.9b00224
This record's URL: https://www.repository.cam.ac.uk/handle/1810/294021
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