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dc.contributor.authorZilberzwige-Tal, Shaien
dc.contributor.authorLevin, Aviaden
dc.contributor.authorToprakcioglu, Zenonen
dc.contributor.authorKnowles, Tuomasen
dc.contributor.authorGazit, Ehuden
dc.contributor.authorElbaz, Johannen
dc.date.accessioned2019-06-25T23:30:58Z
dc.date.available2019-06-25T23:30:58Z
dc.identifier.issn1613-6810
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/294022
dc.description.abstractIn nature, intracellular microcompartments have evolved to allow the simultaneous execution of tightly regulated complex processes within a controlled environment. This architecture serves as the blueprint for the construction of a wide array of artificial cells. However, such systems are inadequate in their ability to confine and sequentially control multiple central dogma activities (transcription, translation, and post-translational modifications) resulting in a limited production of complex biomolecules. Here, an artificial cell-on-a-chip comprising hierarchical compartments allowing the processing and transport of products from transcription, translation, and post-translational modifications through connecting channels is designed and fabricated. This platform generates a tightly controlled system, yielding directly a purified modified protein, with the potential to produce proteoform of choice. Using this platform, the full ubiquitinated form of the Parkinson's disease-associated α-synuclein is generated starting from DNA, in a single device. By bringing together all central dogma activities in a single controllable platform, this approach will open up new possibilities for the synthesis of complex targets, will allow to decipher diverse molecular mechanisms in health and disease and to engineer protein-based materials and pharmaceutical agents.
dc.description.sponsorshipThis study was supported by the Adelis Foundation. S.Z.T. was supported by the Daniel Turnberg Travel Fellowship.
dc.languageengen
dc.publisherWiley
dc.rightsAll rights reserved
dc.subjectaptamersen
dc.subjectcell freeen
dc.subjectmicrofluidicsen
dc.subjectpost-translation modificationsen
dc.subjectsynthetic biologyen
dc.titleProgrammable On-Chip Artificial Cell Producing Post-Translationally Modified Ubiquitinated Protein.en
dc.typeArticle
prism.publicationNameSmallen
dc.identifier.doi10.17863/CAM.41130
dcterms.dateAccepted2019-06-16en
rioxxterms.versionofrecord10.1002/smll.201901780en
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2019-06-16en
dc.contributor.orcidLevin, Aviad [0000-0002-3949-1033]
dc.contributor.orcidToprakcioglu, Zenon [0000-0003-1964-8432]
dc.contributor.orcidKnowles, Tuomas [0000-0002-7879-0140]
dc.identifier.eissn1613-6829
rioxxterms.typeJournal Article/Reviewen
cam.issuedOnline2019-06-17en
cam.orpheus.successThu Jan 30 10:44:17 GMT 2020 - Embargo updated*
rioxxterms.freetoread.startdate2020-06-17


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