Fluids released from subducted slabs impart characteristic geochemical signatures on volcanic arc magmas and residual slabs transported into the deeper mantle. Yet, the sources and transport mechanisms of trace elements released from the slab are speculative. We investigate fluids released from subducted slabs from the perspective of 238U/235U and radiogenic Pb isotope ratios in lavas from the Izu volcanic arc in the Pacific ocean. Izu arc lavas are fluid-dominated end-member type magmas that allow a close characterization of slab fluids. The Izu arc lavas have low 238U/235U ratios compared to the bulk Earth and mid-ocean ridge basalt (MORB). The low 238U/235U (δ238U = -0.46 to -0.33 ‰, where δ238U = 238U/235Usample/ 238U/235UCRM145 -1) is associated with slab-derived fluids low in Th/U that are added to the magma sources. The radiogenic Pb isotope ratios of the lavas form an array between ‘Indian’ type MORB and subducting sediments that is inconsistent with fluids derived from the altered mafic oceanic crust (AMOC). We infer that ‘fluid-mobile’ elements, including U and Pb are mobilized from largely unaltered, deeper sections of the mafic crust by migrating fluids that are derived from the dehydration of underlying serpentinites. Uranium is only fluid-mobile as UVI and needs to be oxidised from predominant UIV in unaltered magmatic rocks in order to be mobilised by fluids. Uranium isotope fractionation of ~ 0.2 ‰ in δ238U during this process is required to generate the low 238U/235U in the fluids. We propose that channelized fluid flow through the metamorphosed sheeted dyke and gabbroic sections of the mafic crust locally oxidizes and mobilizes U. We suggest that U isotope fractionation occurs within the fluid channels and is related to equilibrium isotope fractionation during the oxidation of U and the incorporation of UIV into secondary phases such as epidote, apatite and zircon that grow within the channels. These phases are predicted to carry isotopically heavy U into the deeper mantle beyond subduction zones. The δ238U is thus tracing the dehydration process of subducting slabs. Similar observations have been made for other, ‘stable isotope’ systems in different arcs and subduction-related metamorphic rocks, thus highlighting their potential for studying processes occurring within the slabs during subduction. This information is essential for understanding and the partitioning of elements between subducted slabs and the mantle wedge and constraining the role of subduction zones in global geochemical cycles.