MicroRNA-155 is essential for the optimal proliferation and survival of plasmablast B cells.
Life science alliance
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Arbore, G., Henley, T., Biggins, L., Andrews, S. R., Vigorito, E., Turner, M., & Leyland, R. (2019). MicroRNA-155 is essential for the optimal proliferation and survival of plasmablast B cells.. Life science alliance, 2 (3)https://doi.org/10.26508/lsa.201800244
A fast antibody response can be critical to contain rapidly dividing pathogens. This can be achieved by the expansion of antigen-specific B-cells in response to T-cell help followed by differentiation into plasmablasts. MicroRNA-155 (miR-155) is required for optimal T-cell dependent extrafollicular responses via regulation of PU.1, though the cellular processes underlying this defect are largely unknown. Here we show that miR-155 regulates the early expansion of B-blasts and later on the survival and proliferation of plasmablasts in a B-cell intrinsic manner, by tracking antigen-specific B cells in vivo since the onset of antigen stimulation. In agreement, comparative analysis of the transcriptome of miR-155 sufficient and deficient plasmablasts at the peak of the response showed that the main processes regulated by miR-155 were DNA metabolic process, DNA replication and cell cycle. Thus, miR-155 controls the extent of the extra-follicular response by regulating the survival and proliferation of B-blasts, plasmablasts and, consequently, antibody production.
B-Lymphocyte Subsets, Plasma Cells, Animals, Mice, Knockout, Mice, MicroRNAs, Immunophenotyping, Lymphocyte Activation, Cell Proliferation, Cell Survival, Antibody Formation, Biomarkers
Medical Research Council (MC_UU_00002/4)
External DOI: https://doi.org/10.26508/lsa.201800244
This record's URL: https://www.repository.cam.ac.uk/handle/1810/294762
Attribution 4.0 International
Licence URL: https://creativecommons.org/licenses/by/4.0/