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dc.contributor.authorArbore, Giuseppina
dc.contributor.authorHenley, Tom
dc.contributor.authorBiggins, Laura
dc.contributor.authorAndrews, Simon Richard
dc.contributor.authorVigorito, Elena
dc.contributor.authorTurner, Martin
dc.contributor.authorLeyland, Rebecca
dc.date.accessioned2019-07-18T23:30:14Z
dc.date.available2019-07-18T23:30:14Z
dc.date.issued2019-06
dc.identifier.issn2575-1077
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/294762
dc.description.abstractA fast antibody response can be critical to contain rapidly dividing pathogens. This can be achieved by the expansion of antigen-specific B cells in response to T-cell help followed by differentiation into plasmablasts. MicroRNA-155 (miR-155) is required for optimal T-cell-dependent extrafollicular responses via regulation of PU.1, although the cellular processes underlying this defect are largely unknown. Here, we show that miR-155 regulates the early expansion of B-blasts and later on the survival and proliferation of plasmablasts in a B-cell-intrinsic manner, by tracking antigen-specific B cells in vivo since the onset of antigen stimulation. In agreement, comparative analysis of the transcriptome of miR-155-sufficient and miR-155-deficient plasmablasts at the peak of the response showed that the main processes regulated by miR-155 were DNA metabolic process, DNA replication, and cell cycle. Thus, miR-155 controls the extent of the extrafollicular response by regulating the survival and proliferation of B-blasts, plasmablasts and, consequently, antibody production.
dc.format.mediumElectronic-Print
dc.languageeng
dc.publisherLife Science Alliance, LLC
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectB-Lymphocyte Subsets
dc.subjectPlasma Cells
dc.subjectAnimals
dc.subjectMice, Knockout
dc.subjectMice
dc.subjectMicroRNAs
dc.subjectImmunophenotyping
dc.subjectLymphocyte Activation
dc.subjectCell Proliferation
dc.subjectCell Survival
dc.subjectAntibody Formation
dc.subjectBiomarkers
dc.titleMicroRNA-155 is essential for the optimal proliferation and survival of plasmablast B cells.
dc.typeArticle
prism.issueIdentifier3
prism.publicationDate2019
prism.publicationNameLife Sci Alliance
prism.volume2
dc.identifier.doi10.17863/CAM.41859
dcterms.dateAccepted2019-04-30
rioxxterms.versionofrecord10.26508/lsa.201800244
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2019-06
dc.contributor.orcidArbore, Giuseppina [0000-0002-8318-7557]
dc.contributor.orcidAndrews, Simon Richard [0000-0002-5006-3507]
dc.contributor.orcidVigorito, Elena [0000-0001-6230-3849]
dc.contributor.orcidTurner, Martin [0000-0002-3801-9896]
dc.contributor.orcidLeyland, Rebecca [0000-0002-0310-381X]
dc.identifier.eissn2575-1077
rioxxterms.typeJournal Article/Review
pubs.funder-project-idMedical Research Council (MC_UU_00002/4)
cam.issuedOnline2019-05-16


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International