Fabrication of free standing collagen membranes by pulsed-electrophoretic deposition.
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Barrett, D., Linley, M. D., Best, S., & Cameron, R. (2019). Fabrication of free standing collagen membranes by pulsed-electrophoretic deposition.. Biofabrication, 11 (4), 045017. https://doi.org/10.1088/1758-5090/ab331d
This work reports an important new development in the production of collagen membranes, based on pulsed electrophoretic deposition (P-EPD), suitable for a wide range of biomedical applications. Collagen membranes are of great interest as a biomaterial and in a range of other industries, though current production techniques suffer from limitations with scaling up, homogeneity, and complex shapes. P-EPD can be used to rapidly create detachable, large-area, homogeneous products with controlled thickness in a wide variety of shapes. We provide a new understanding of the influence of a range of parameters (pulse width, voltage, duty cycle, solvent additions) and their effects on membrane structure. Characterisation by AFM, SEM, and cryoSEM revealed the ability to produce dense, structurally defect-free membranes, and significantly, we show and discuss the ability to produce thicker membranes by sequential deposition without seeing a corresponding increase in cell electrical resistance. We anticipate this novel, rapid, and controllable method for the production of collagen membranes to be of interest for a wide range of fields.
Animals, Cattle, Collagen, Membranes, Artificial, Hydrogels, Solvents, Imaging, Three-Dimensional, Electrophoresis, Static Electricity
The authors wish to acknowledge the support of theEngineering and Physical Sciences Research Council(EPSRC)grants EP/K503009/1, EP/J500380/1, EP/L504920/1, EP/M506485/1, and EP/M508007/1,Geistlich Pharma AG, and the European ResearchCouncil(ERC)Advanced Grant 320598 3D-E
European Research Council (320598)
External DOI: https://doi.org/10.1088/1758-5090/ab331d
This record's URL: https://www.repository.cam.ac.uk/handle/1810/294916
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