Fabrication of free standing collagen membranes by pulsed-electrophoretic deposition.
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Peer-reviewed
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This work reports an important new development in the production of collagen membranes, based on pulsed electrophoretic deposition (P-EPD), suitable for a wide range of biomedical applications. Collagen membranes are of great interest as a biomaterial and in a range of other industries, though current production techniques suffer from limitations with scaling up, homogeneity, and complex shapes. P-EPD can be used to rapidly create detachable, large-area, homogeneous products with controlled thickness in a wide variety of shapes. We provide a new understanding of the influence of a range of parameters (pulse width, voltage, duty cycle, solvent additions) and their effects on membrane structure. Characterisation by AFM, SEM, and cryoSEM revealed the ability to produce dense, structurally defect-free membranes, and significantly, we show and discuss the ability to produce thicker membranes by sequential deposition without seeing a corresponding increase in cell electrical resistance. We anticipate this novel, rapid, and controllable method for the production of collagen membranes to be of interest for a wide range of fields.
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1758-5090
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EPSRC (1365562)
Engineering and Physical Sciences Research Council (EP/K503009/1)
Engineering and Physical Sciences Research Council (EP/L504920/1)
Engineering and Physical Sciences Research Council (EP/M506485/1)
Engineering and Physical Sciences Research Council (EP/M508007/1)