Single-cell genome-wide bisulfite sequencing for assessing epigenetic heterogeneity.
Smallwood, Sébastien A
Lee, Heather J
Nature Publishing Group
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Smallwood, S. A., Lee, H. J., Angermueller, C., Krueger, F., Saadeh, H., Peat, J., Andrews, S. R., et al. (2014). Single-cell genome-wide bisulfite sequencing for assessing epigenetic heterogeneity.. Nat Methods, 11 (8), 817-820. https://doi.org/10.1038/nmeth.3035
We report a single-cell bisulfite sequencing (scBS-seq) method that can be used to accurately measure DNA methylation at up to 48.4% of CpG sites. Embryonic stem cells grown in serum or in 2i medium displayed epigenetic heterogeneity, with '2i-like' cells present in serum culture. Integration of 12 individual mouse oocyte datasets largely recapitulated the whole DNA methylome, which makes scBS-seq a versatile tool to explore DNA methylation in rare cells and heterogeneous populations.
Animals, DNA Methylation, Epigenesis, Genetic, Genome, Mice, Sulfites
This work was supported by the UK Biotechnology and Biological Sciences Research Council grant BB/J004499/1, UK Medical Research Council grant MR/K011332/1, Wellcome Trust award 095645/Z/11/Z and EU FP7 EpiGeneSys and BLUEPRINT.
External DOI: https://doi.org/10.1038/nmeth.3035
This record's URL: https://www.repository.cam.ac.uk/handle/1810/295444
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