Single-cell genome-wide bisulfite sequencing for assessing epigenetic heterogeneity.
Accepted version
Peer-reviewed
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Repository DOI
Change log
Authors
Smallwood, Sébastien A
Lee, Heather J
Angermueller, Christof
Krueger, Felix
Saadeh, Heba
Abstract
We report a single-cell bisulfite sequencing (scBS-seq) method that can be used to accurately measure DNA methylation at up to 48.4% of CpG sites. Embryonic stem cells grown in serum or in 2i medium displayed epigenetic heterogeneity, with '2i-like' cells present in serum culture. Integration of 12 individual mouse oocyte datasets largely recapitulated the whole DNA methylome, which makes scBS-seq a versatile tool to explore DNA methylation in rare cells and heterogeneous populations.
Description
Keywords
Animals, DNA Methylation, Epigenesis, Genetic, Genome, Mice, Sulfites
Journal Title
Nat Methods
Conference Name
Journal ISSN
1548-7091
1548-7105
1548-7105
Volume Title
11
Publisher
Nature Publishing Group
Publisher DOI
Rights
All rights reserved
Sponsorship
This work was supported by the UK Biotechnology and Biological Sciences Research Council grant BB/J004499/1, UK Medical Research Council grant MR/K011332/1, Wellcome Trust award 095645/Z/11/Z and EU FP7 EpiGeneSys and BLUEPRINT.