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Establishing the Japan-Store House of Animal Radiobiology Experiments (J-SHARE), a large-scale necropsy and histopathology archive providing international access to important radiobiology data

Accepted version
Peer-reviewed

Type

Article

Change log

Authors

Morioka, Takamitsu 
Blyth, Benjamin 
Imaoka, Tatsuhiko 
Nishimura, Mayumi 

Abstract

Purpose: Projects evaluating the effects of radiation, within the National Institutes of Quantum and Radiological Science and Technology (QST), National Institute of Radiological Sciences (NIRS), have focused on risk analyses for life shortening and cancer prevalence using laboratory animals. Genetic and epigenetic alterations in radiation-induced tumors have been also analyzed, with the aim of better understanding mechanisms of radiation carcinogenesis. As well as the economic and practical limitations of repeating such large-scale experiments, ethical considerations make it vital that we store and share the pathological data and samples of the animal experiments for future use. We are now constructing such an archive called the Japan-Storehouse of Animal Radiobiology Experiments (J-SHARE).

Methods: J-SHARE records include information such as detailed experimental protocols, necropsy records and photographs of organs at necropsy. For each animal organs and tumor tissues are dissected, and parts are stored as frozen samples at -80 ˚C. Samples fixed with formalin are also embedded in paraffin blocks for histopathological analyses. Digital copies of stained tissues are being systematically saved using a virtual slide system linked to original records by barcodes. Embedded and frozen tissues are available for molecular analysis. Conclusion: Similar archive systems for radiation biology have been also under construction in the USA and Europe, the Northwestern University Radiation Archive (NURA), and STORE at the BfS, respectively. The J-SHARE will be linked with the sister-archives and made available for collaborative research to institutions and universities all over the world.

Description

Keywords

Animal experiments, archive, database, pathology, tissue specimen, Access to Information, Animal Experimentation, Animals, Archives, Carcinogenesis, Databases, Factual, Histology, Humans, Japan, Medical Records, Mice, Neoplasms, Neoplasms, Radiation-Induced, Program Development, Radiobiology, Research, Research Design, Risk Assessment

Journal Title

International Journal of Radiation Biology

Conference Name

Journal ISSN

0955-3002
1362-3095

Volume Title

Publisher

Taylor & Francis

Rights

All rights reserved