Genome architecture and stability in the Saccharomyces cerevisiae knockout collection.
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Authors
Selivanova, Alexandra
Wang, Siyue
Zhu, Jin
Klein-Lavi, Shir
Gordon, Molly
Meirman, Roi
Ayestaran, Iñigo
Salguero, Israel
Sharan, Roded
Li, Rong
Kupiec, Martin
Publication Date
2019-09-11Journal Title
Nature
ISSN
0028-0836
Publisher
Nature Publishing Group
Volume
573
Issue
7774
Pages
416-420
Language
eng
Type
Article
This Version
AM
Physical Medium
Print-Electronic
Metadata
Show full item recordCitation
Puddu, F., Herzog, M., Selivanova, A., Wang, S., Zhu, J., Klein-Lavi, S., Gordon, M., et al. (2019). Genome architecture and stability in the Saccharomyces cerevisiae knockout collection.. Nature, 573 (7774), 416-420. https://doi.org/10.1038/s41586-019-1549-9
Abstract
While identification and analysis of genes affecting genome stability have
traditionally relied on reporter assays, whole-genome sequencing technologies now enable, in principle, direct measurements of genome instability globally and at scale. Here, we have surveyed the Saccharomyces cerevisiae gene knockout collection by sequencing the whole genomes of its strains, and characterized genomic changes caused by the absence of essentially any one of the non-essential yeast genes. Analysing this dataset (http://sgv.gurdon.cam.ac.uk) reveals genes affecting repetitive-element maintenance or mutagenesis, highlights cross-talks between nuclear and mitochondrial genome stability, and shows how strains have adapted to
loss of non-essential genes.
Keywords
Saccharomyces cerevisiae, Genomic Instability, Adaptation, Biological, Genome, Fungal, Genome, Mitochondrial, Gene Knockout Techniques, Whole Genome Sequencing
Sponsorship
Wellcome Trust (101126/Z/13/Z)
Cancer Research UK (A18796)
Wellcome Trust (206388/Z/17/Z)
Cancer Research UK (C6946/A24843)
Wellcome Trust (203144/Z/16/Z)
Identifiers
External DOI: https://doi.org/10.1038/s41586-019-1549-9
This record's URL: https://www.repository.cam.ac.uk/handle/1810/295816
Rights
All rights reserved