Quantitative comparative analysis of human erythrocyte surface proteins between individuals from two genetically distinct populations.
Authors
Ravenhill, Benjamin J
Kanjee, Usheer
Ahouidi, Ambroise
Veiga Nobre, Luis
Goldberg, Jonathan M
Antrobus, Robin
Dieye, Tandakha
Duraisingh, Manoj T
Publication Date
2019-01Journal Title
Communications biology
ISSN
2399-3642
Volume
2
Pages
350
Language
eng
Type
Article
This Version
AM
Physical Medium
Electronic-eCollection
Metadata
Show full item recordCitation
Ravenhill, B. J., Kanjee, U., Ahouidi, A., Veiga Nobre, L., Williamson, J., Goldberg, J. M., Antrobus, R., et al. (2019). Quantitative comparative analysis of human erythrocyte surface proteins between individuals from two genetically distinct populations.. Communications biology, 2 350. https://doi.org/10.1038/s42003-019-0596-y
Abstract
Red blood cells (RBC) play a critical role in oxygen transport, and are the focus of important diseases including malaria and the haemoglobinopathies. Proteins at the RBC surface can determine susceptibility to disease, however previous studies classifying the RBC proteome have not used specific strategies directed at enriching cell surface proteins. Furthermore, there has been no systematic analysis of variation in abundance of RBC surface proteins between genetically disparate human populations. These questions are important to inform not only basic RBC biology but additionally to identify novel candidate receptors for malarial parasites. Here, we use ‘Plasma membrane profiling’ and tandem mass tag-based mass spectrometry to enrich and quantify primary RBC cell surface proteins from two sets of nine donors from the UK or Senegal. We define a RBC surface proteome and identify potential Plasmodium receptors based on either diminished protein abundance, or increased variation in RBC from West African individuals.
Keywords
Erythrocytes, Erythrocyte Membrane, Humans, Membrane Proteins, Proteome, Proteomics, Systems Biology
Sponsorship
WELLCOME TRUST (108070/Z/15/Z)
Wellcome Trust (100140/Z/12/Z)
Embargo Lift Date
2022-08-19
Identifiers
External DOI: https://doi.org/10.1038/s42003-019-0596-y
This record's URL: https://www.repository.cam.ac.uk/handle/1810/295993
Rights
All rights reserved