Actomyosin controls planarity and folding of epithelia in response to compression.
Wyatt, Tom PJ
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Wyatt, T. P., Fouchard, J., Lisica, A., Khalilgharibi, N., Baum, B., Recho, P., Kabla, A., & et al. (2020). Actomyosin controls planarity and folding of epithelia in response to compression.. Nature materials, 19 (1), 109-117. https://doi.org/10.1038/s41563-019-0461-x
Throughout embryonic development and adult life, epithelia are subjected to compressive deformations. While these have been shown to trigger mechanosensitive responses such as cell extrusion and differentiation, which span tens of minutes, little is known about how epithelia adapt to compression over shorter timescales. Here, using suspended epithelia, we uncover the immediate response of epithelial tissues to the application of in-plane compressive strains (5–80%). We show that fast compression induces tissue buckling followed by actomyosin-dependent tissue flattening that erases the buckle within tens of seconds, in both mono- and multi-layered epithelia. Strikingly, we identify a well-defined limit to this response, so that stable folds form in the tissue when compressive strains exceed a ‘buckling threshold’ of ~35%. A combination of experiment and modelling shows that this behaviour is orchestrated by adaptation of the actomyosin cytoskeleton as it re-establishes tissue tension following compression. Thus, tissue pre-tension allows epithelia to both buffer against deformation and sets their ability to form and retain folds during morphogenesis.
Epithelium, Cytoskeleton, Epithelial Cells, Animals, Dogs, Actomyosin, Cadherins, Green Fluorescent Proteins, Microscopy, Confocal, Morphogenesis, Viscosity, Compressive Strength, Elasticity, Stress, Mechanical, Models, Biological, Madin Darby Canine Kidney Cells
T.P.J.W. and N.K. were part of the EPSRC funded doctoral training programme CoMPLEX. J.F. and P.R. were funded by BBSRC grants (nos. BB/M003280 and BB/M002578) to G.T.C. and A.J.K. N.K. was funded by the Rosetrees Trust and the UCL Graduate School through a UCL Overseas Research Scholarship. A.L. was supported by an EMBO long-term post-doctoral fellowship. B.B. was supported by UCL, a BBSRC project grant (no. BB/K009001/1) and a CRUK programme grant (no. 17343). T.P.J.W., J.F., N.K., A.L. and G.T.C. were supported by a consolidator grant from the European Research Council to G.T.C. (MolCellTissMech, agreement no. 647186).
External DOI: https://doi.org/10.1038/s41563-019-0461-x
This record's URL: https://www.repository.cam.ac.uk/handle/1810/296258
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