FeII4L4 Tetrahedron Binds to Nonpaired DNA Bases.
Greenhalgh, Ryan D
Journal of the American Chemical Society
American Chemical Society
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Zhu, J., Haynes, C., Kieffer, M., Greenfield, J., Greenhalgh, R. D., Nitschke, J., & Keyser, U. (2019). FeII4L4 Tetrahedron Binds to Nonpaired DNA Bases.. Journal of the American Chemical Society, 141 (29), 11358-11362. https://doi.org/10.1021/jacs.9b03566
A water-soluble self-assembled supramolecular FeII4L4 tetrahedron binds to single stranded DNA, mismatched DNA base pairs, and three-way DNA junctions. Binding of the coordination cage quenches fluorescent labels on the DNA strand, which provides an optical means to detect the interaction and allows the position of the binding site to be gauged with respect to the fluorescent label. Utilizing the quenching and binding properties of the coordination cage, we developed a simple and rapid detection method based on fluorescence quenching to detect unpaired bases in double-stranded DNA.
The authors acknowledge support from the UK Engineering and Physical Sciences Research Council (EPSRC EP/M008258/1, EPSRC EP/P027067/1, and EPSRC EP/L015978/1). This project has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement No 642192
ECH2020 EUROPEAN RESEARCH COUNCIL (ERC) (647144)
EPSRC (via University of Manchester) (EP/P027067/1)
European Commission (642192)
External DOI: https://doi.org/10.1021/jacs.9b03566
This record's URL: https://www.repository.cam.ac.uk/handle/1810/296350
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