Repository logo
 

Using Tetracysteine-Tagged TDP-43 with a Biarsenical Dye To Monitor Real-Time Trafficking in a Cell Model of Amyotrophic Lateral Sclerosis.

Accepted version
Peer-reviewed

Change log

Authors

Ng, Janice SW 
Hanspal, Maya A 
Matharu, Naunehal S 
Barros, Teresa P 

Abstract

TAR DNA-binding protein 43 (TDP-43) has been identified as the major constituent of the proteinaceous inclusions that are characteristic of most forms of amyotrophic lateral sclerosis (ALS) and ubiquitin positive frontotemporal lobar degeneration (FTLD). Wild type TDP-43 inclusions are a pathological hallmark of >95% of patients with sporadic ALS and of the majority of familial ALS cases, and they are also found in a significant proportion of FTLD cases. ALS is the most common form of motor neuron disease, characterized by progressive weakness and muscular wasting, and typically leads to death within a few years of diagnosis. To determine how the translocation and misfolding of TDP-43 contribute to ALS pathogenicity, it is crucial to define the dynamic behavior of this protein within the cellular environment. It is therefore necessary to develop cell models that allow the location of the protein to be defined. We report the use of TDP-43 with a tetracysteine tag for visualization using fluorogenic biarsenical compounds and show that this model displays features of ALS observed in other cell models. We also demonstrate that this labeling procedure enables live-cell imaging of the translocation of the protein from the nucleus into the cytosol.

Description

Keywords

Amino Acid Sequence, Amyotrophic Lateral Sclerosis, Cell Line, Tumor, Cell Nucleus, Cysteine, Cytosol, DNA-Binding Proteins, Electroporation, Fluoresceins, Fluorescent Dyes, Frontotemporal Lobar Degeneration, Humans, Kinetics, Models, Biological, Optical Imaging, Organometallic Compounds, Protein Transport, Sequence Tagged Sites, Time-Lapse Imaging, Transfection

Journal Title

Biochemistry

Conference Name

Journal ISSN

0006-2960
1520-4995

Volume Title

58

Publisher

American Chemical Society (ACS)

Rights

All rights reserved
Sponsorship
Wellcome Trust (094425/Z/10/Z)