SEQUENCING AND ANALYSIS OF PUTATIVE 3D-4H RING CYCLASE GENE lndF OF STREPTOMYCES GLOBISPORUS 1912 LANDOMYCIN E BIOSYNTHETIC GENE CLUSTER
Visnyk of Lviv University, Biology Series
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Ostash, B., Rebets, Y., Samborskyy, M., Salas, J., & Fedorenko, V. (2003). SEQUENCING AND ANALYSIS OF PUTATIVE 3D-4H RING CYCLASE GENE lndF OF STREPTOMYCES GLOBISPORUS 1912 LANDOMYCIN E BIOSYNTHETIC GENE CLUSTER. Visnyk of Lviv University, Biology Series, (32), 84-91. http://prima.lnu.edu.ua/faculty/biologh/wis/english.htm
DNA fragment of landomycin E biosynthesis gene cluster 700 bp in size has been completely sequenced. 3?-end of lndE (oxygenase) was identified, 5?-end of lndA (ketosynthase) and entire ORF for previously not sequenced lanF homologue, lndF. Analysis of lndF putative translation product revealed that it is highly conservative in comparison with other known cyclases from antibiotic biosynthesis gene clusters. Unlike urdamycin, jadomycin biosynthetic clusters, lndF and lndA are uncoupled, as well as genes lanF and lanA. Genes lndF and lndA are not preceded by direct or inverted repeats, putative sites for binding of transcriptional activator LndI. In contrast, lanF is flanked at it's 5?-end by three direct repeats, possible target for regulatory protein LanI.
Streptomyces, angucyclines, cyclases, landomycin E
L. Castro is acknowledged for help in sequencing. This work was supported by INTAS grant YSF 00-208 to B.O.
External link: http://prima.lnu.edu.ua/faculty/biologh/wis/english.htm
This record's URL: https://www.repository.cam.ac.uk/handle/1810/296692