Tumor-derived TGF-beta inhibits mitochondrial respiration to suppress IFN-gamma production by human CD4+ T cells
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Authors
Hess, Christophe
Journal Title
Science Signaling
ISSN
1937-9145
Publisher
American Association for the Advancement of Science
Type
Article
This Version
AM
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Hess, C. (2019). Tumor-derived TGF-beta inhibits mitochondrial respiration to suppress IFN-gamma production by human CD4+ T cells. Science Signaling https://doi.org/10.1126/scisignal.aav3334
Abstract
TGF-beta is produced by tumors, and increased levels are associated with poor survival. Functionally, TGF-beta-mediated suppression of anti-tumor T cell responses contributes to tumor growth and survival. However, TGF-beta can also have tumor suppressive activity, and dissecting cell-type specific molecular effects may decisively inform therapeutic strategies targeting this cytokine. Here we investigated the mechanisms involved in suppression of a key anti-tumor CD4+ T cell function –interferon-gamma (IFN-gamma) production– by tumor-derived TGF-beta in human peripheral and tumor-associated lymphocytes. Suppression required expression and phosphorylation of TGF-beta signaling proteins (Smad proteins), but not their nuclear translocation, and was dependent on oxygen availability –suggesting a metabolic basis. Indeed, Smad proteins were detected in mitochondria of CD4+ T cells, where they were phosphorylated upon TGF-beta exposure. Phosphorylated Smad proteins were also detected ex vivo in mitochondria of tumor-associated lymphocytes. TGF-beta treatment significantly impaired ATP-coupled respiration of CD4+ T cells, and specifically mitochondrial complex V (ATP-Synthase) activity. Finally, inhibition of ATP-Synthase per se was sufficient to impair IFN-gamma production. These results demonstrate that TGF-beta targets T cell metabolism directly, thus diminishing their function (metabolic paralysis), with relevance to human anti-tumor immunity.
Sponsorship
This work was
supported by the Roche postdoctoral fellowship program and University of Basel research
funds (to S.D.) and by Swiss National Science Foundation grants 310030_153059 (to C.H.) and
323530-139181 (to M.F.)
Identifiers
External DOI: https://doi.org/10.1126/scisignal.aav3334
This record's URL: https://www.repository.cam.ac.uk/handle/1810/297264
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