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Research data supporting "Independent effects on cellular and humoral immune responses underlie genotype-by-genotype interactions between Drosophila and parasitoids"


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Type

Dataset

Change log

Authors

Castanho Barata Leitao, Alexandre  ORCID logo  https://orcid.org/0000-0001-9004-1831
Bian, Xueni 
P. Day, Jonathan 
Pitton, Simone 
Demir, Esref 

Description

Datasets and scripts to generate each figure panel are divided in separate folders. Scripts generate and save each figure as a PDF file. Statistics are generated at the end of each script. Folders: Fig1A – The encapsulation phenotype is recorded for individual larva per treatment and genotype. Fig1B – The melanization phenotype is recorded for individual larva per treatment and genotype. Fig2 – The number of plasmatocytes and lamellocytes is recorded from pool of 6 larvae per treatment and genotype. Fig3A – Gene expression is recorded as raw Ct values from qPCR for housekeeping gene and test gene per treatment and genotype. Fig3B – Phenoloxidase activity is recorded as raw absorbance value per time point, treatment and genotype. FigS1 – The encapsulation phenotype is recorded per vial with 40 transferred larvae before infection.

Version

Software / Usage instructions

All scripts were written in R version 3.3.3

Keywords

D. melanogaster, L. boulardi, Infection, Immunity

Publisher

Sponsorship
Natural Environment Research Council (NE/P00184X/1)
Relationships
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