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A robust human norovirus replication model in zebrafish larvae.

Published version
Peer-reviewed

Type

Article

Change log

Authors

Conceição-Neto, Nádia 
Maes, Jan 

Abstract

Human noroviruses (HuNoVs) are the most common cause of foodborne illness, with a societal cost of $60 billion and 219,000 deaths/year. The lack of robust small animal models has significantly hindered the understanding of norovirus biology and the development of effective therapeutics. Here we report that HuNoV GI and GII replicate to high titers in zebrafish (Danio rerio) larvae; replication peaks at day 2 post infection and is detectable for at least 6 days. The virus (HuNoV GII.4) could be passaged from larva to larva two consecutive times. HuNoV is detected in cells of the hematopoietic lineage and the intestine, supporting the notion of a dual tropism. Antiviral treatment reduces HuNoV replication by >2 log10, showing that this model is suited for antiviral studies. Zebrafish larvae constitute a simple and robust replication model that will largely facilitate studies of HuNoV biology and the development of antiviral strategies.

Description

Keywords

Animals, Antiviral Agents, Caliciviridae Infections, Foodborne Diseases, Gastroenteritis, Host Microbial Interactions, Humans, Larva, Metagenomics, Models, Animal, Norovirus, Virus Cultivation, Virus Replication, Zebrafish

Journal Title

PLoS Pathog

Conference Name

Journal ISSN

1553-7366
1553-7374

Volume Title

15

Publisher

Public Library of Science (PLoS)
Sponsorship
Wellcome Trust (097997/Z/11/Z)
Wellcome Trust (207498/Z/17/Z)
European Commission (608765)
Wellcome Trust (207498/Z/17/Z)