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dc.contributor.authorSokol, Katarzyna Pen
dc.contributor.authorRobinson, William Een
dc.contributor.authorOliveira, Ana Ren
dc.contributor.authorZacarias, Soniaen
dc.contributor.authorLee, Chong-Yongen
dc.contributor.authorMadden, Christopheren
dc.contributor.authorBassegoda, Arnauen
dc.contributor.authorHirst, Judyen
dc.contributor.authorPereira, Inês ACen
dc.contributor.authorReisner, Erwinen
dc.date.accessioned2019-10-30T00:31:14Z
dc.date.available2019-10-30T00:31:14Z
dc.date.issued2019-11en
dc.identifier.issn0002-7863
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/298222
dc.description.abstractThe biological formate hydrogenlyase (FHL) complex links a formate dehydrogenase (FDH) to a hydrogenase (H2ase) and produces H2 and CO2 from formate via mixed-acid fermentation in Escherichia coli. Here, we describe an electrochemical and a colloidal semi-artificial FHL system that consists of an FDH and a H2ase immobilized on conductive indium tin oxide (ITO) as an electron relay. These in vitro systems benefit from the efficient wiring of a highly active enzyme pair and allow for the reversible conversion of formate to H2 and CO2 under ambient temperature and pressure. The hybrid systems provide a template for the design of synthetic catalysts and surpass the FHL complex in vivo by storing and releasing H2 on demand by interconverting CO2/H2 and formate with minimal bias in either direction.
dc.description.sponsorshipERC Consolidator Grant “MatEnSAP” (682833), BBSRC (BB/J000124/1, BB/I026367/1), EPSRC (EP/L015978/1, EP/G037221/1, nanoDTC and a DTA studentship to K.P.S.), Marie Curie IntraEuropean Fellowship (PIEFGA- 2013-625034), Fundação para a Ciência e Tecnologia (Portugal) fellowship SFRH/BD/116515/2016, grants PTDC/BIAMIC/ 2723/2014,
dc.format.mediumPrint-Electronicen
dc.languageengen
dc.publisherAmerican Chemical Society
dc.rightsAll rights reserved
dc.rights.uri
dc.titleReversible and Selective Interconversion of Hydrogen and Carbon Dioxide into Formate by a Semiartificial Formate Hydrogenlyase Mimic.en
dc.typeArticle
prism.endingPage17502
prism.issueIdentifier44en
prism.publicationDate2019en
prism.publicationNameJournal of the American Chemical Societyen
prism.startingPage17498
prism.volume141en
dc.identifier.doi10.17863/CAM.45274
dcterms.dateAccepted2019-10-22en
rioxxterms.versionofrecord10.1021/jacs.9b09575en
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2019-11en
dc.contributor.orcidOliveira, Ana R [0000-0001-7828-4152]
dc.contributor.orcidHirst, Judy [0000-0001-8667-6797]
dc.contributor.orcidPereira, Inês AC [0000-0003-3283-4520]
dc.contributor.orcidReisner, Erwin [0000-0002-7781-1616]
dc.identifier.eissn1520-5126
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idECH2020 EUROPEAN RESEARCH COUNCIL (ERC) (682833)
pubs.funder-project-idEPSRC (EP/L015978/1)
pubs.funder-project-idEPSRC (EP/G037221/1)
pubs.funder-project-idBBSRC (BB/J000124/1)
pubs.funder-project-idBBSRC (BB/I026367/1)
pubs.funder-project-idEuropean Commission (625034)
pubs.funder-project-idMRC (MC_UU_00015/2)
cam.orpheus.successThu Jan 30 10:36:22 GMT 2020 - Embargo updated*
rioxxterms.freetoread.startdate2020-11-30


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