Plant development. Arabidopsis NAC45/86 direct sieve element morphogenesis culminating in enucleation.
Furuta, Kaori Miyashima
Yadav, Shri Ram
De Rybel, Bert
Van Isterdael, Gert
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Furuta, K. M., Yadav, S. R., Lehesranta, S., Belevich, I., Miyashima, S., Heo, J., Vatén, A., et al. (2014). Plant development. Arabidopsis NAC45/86 direct sieve element morphogenesis culminating in enucleation.. Science, 345 (6199), 933-937. https://doi.org/10.1126/science.1253736
Photoassimilates such as sugars are transported through phloem sieve element cells in plants. Adapted for effective transport, sieve elements develop as enucleated living cells. We used electron microscope imaging and three-dimensional reconstruction to follow sieve element morphogenesis in Arabidopsis. We show that sieve element differentiation involves enucleation, in which the nuclear contents are released and degraded in the cytoplasm at the same time as other organelles are rearranged and the cytosol is degraded. These cellular reorganizations are orchestrated by the genetically redundant NAC domain-containing transcription factors, NAC45 and NAC86 (NAC45/86). Among the NAC45/86 targets, we identified a family of genes required for enucleation that encode proteins with nuclease domains. Thus, sieve elements differentiate through a specialized autolysis mechanism.
Arabidopsis, Arabidopsis Proteins, Cell Nucleus, Gene Expression Regulation, Developmental, Gene Expression Regulation, Plant, Imaging, Three-Dimensional, Microscopy, Electron, Morphogenesis, Phloem, Transcription Factors
European Research Council (323052)
Gatsby Charitable Foundation (GAT3395/PR3)
External DOI: https://doi.org/10.1126/science.1253736
This record's URL: https://www.repository.cam.ac.uk/handle/1810/298681
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