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Molecular replication using covalent base-pairs with traceless linkers.

Accepted version
Peer-reviewed

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Authors

Núñez-Villanueva, Diego  ORCID logo  https://orcid.org/0000-0002-1005-1464
Hunter, Christopher A  ORCID logo  https://orcid.org/0000-0002-5182-1859

Abstract

A unique feature of kinetically inert covalent base-pairing is that the nature of the chemical information that is transferred can be modulated by changing the chemical connectivity between the two bases. Formation of esters between phenols and benzoic acids has been used as a base-pairing strategy for sequence information transfer in template-directed synthesis of linear oligomers, but the copy strand produced by this process has the complementary sequence to the template strand. It is possible to form a base-pair between two benzoic acids by using a hydroquinone linker, which is eliminated when the product duplex is hydrolysed. Using this approach, covalent template-directed synthesis was carried out using a benzoic acid 3-mer template to produce an identical copy. This direct replication process was used in iterative rounds of replication leading to an increase of the population of the copied oligomer.

Description

Keywords

3405 Organic Chemistry, 34 Chemical Sciences

Journal Title

Org Biomol Chem

Conference Name

Journal ISSN

1477-0520
1477-0539

Volume Title

17

Publisher

Royal Society of Chemistry (RSC)
Sponsorship
Engineering and Physical Sciences Research Council (EP/P027067/1)
Engineering and Physical Sciences Research Council (EP/P027067/1)