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dc.contributor.authorCaglar, Mustafaen
dc.contributor.authorPandya, Rajen
dc.contributor.authorXiao, Jamesen
dc.contributor.authorFoster, Sarahen
dc.contributor.authorDivitini, Giorgioen
dc.contributor.authorGreenham, Neilen
dc.contributor.authorFranze, Kristianen
dc.contributor.authorRao, Akshayen
dc.contributor.authorKeyser, Ulrichen
dc.date.accessioned2019-11-13T15:14:02Z
dc.date.available2019-11-13T15:14:02Z
dc.identifier.issn1530-6984
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/298869
dc.description.abstractLuminescent semiconductor quantum dots (QDs) have recently been suggested as novel probes for imaging and sensing cell membrane voltages. However, a key bottleneck for their development is a lack of techniques to assess QD responses to voltages generated in the aqueous electrolytic environments typical of biological systems. Even more generally, there have been relatively few efforts to assess the response of QDs to voltage changes in live cells. Here, we develop a platform for monitoring the photoluminescence (PL) response of QDs under AC and DC voltage changes within aqueous ionic environments. We evaluate both traditional CdSe/CdS and more biologically compatible InP/ZnS QDs at a range of ion concentrations to establish their PL/voltage characteristics on chip. Wide-field, few-particle PL measurements with neuronal cells show the QDs can be used to track local voltage changes with greater sensitivity (ΔPL up to twice as large) than state-of-the-art calcium imaging dyes, making them particularly appealing for tracking sub-threshold events. Additional physiological observation studies showed that whilst CdSe/CdS dots have greater PL responses on membrane depolarization, their lower cytotoxicity makes InP/ZnS far more suitable for voltage sensing in living systems. Our results provide a methodology for the rational development of QD voltage sensors and highlight their potential for imaging changes in cell membrane voltage.
dc.description.sponsorshipEPSRC Doctoral Training Award (EP/L016087/1)
dc.publisherAmerican Chemical Society
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleAll-optical detection of neuronal membrane depolarization in live cells using colloidal quantum dotsen
dc.typeArticle
prism.publicationNameNano Letters: a journal dedicated to nanoscience and nanotechnologyen
dc.identifier.doi10.17863/CAM.45924
dcterms.dateAccepted2019-11-05en
rioxxterms.versionofrecord10.1021/acs.nanolett.9b03026en
rioxxterms.versionVoR*
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2019-11-05en
dc.contributor.orcidCaglar, Mustafa [0000-0001-7547-1817]
dc.contributor.orcidPandya, Raj [0000-0003-1108-9322]
dc.contributor.orcidXiao, James [0000-0002-1713-5599]
dc.contributor.orcidDivitini, Giorgio [0000-0003-2775-610X]
dc.contributor.orcidGreenham, Neil [0000-0002-2155-2432]
dc.contributor.orcidFranze, Kristian [0000-0002-8425-7297]
dc.contributor.orcidRao, Akshay [0000-0003-0320-2962]
dc.contributor.orcidKeyser, Ulrich [0000-0003-3188-5414]
dc.identifier.eissn1530-6992
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idEPSRC (1644616)
pubs.funder-project-idEPSRC (EP/M006360/1)
pubs.funder-project-idEPSRC (EP/L016087/1)
cam.issuedOnline2019-11-05en
dc.identifier.urlhttps://pubs.acs.org/doi/10.1021/acs.nanolett.9b03026en


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International