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dc.contributor.authorAbbas, Yassen
dc.contributor.authorBrunel, Lucia G
dc.contributor.authorHollinshead, Michael S
dc.contributor.authorFernando, Ridma C
dc.contributor.authorGardner, Lucy
dc.contributor.authorDuncan, Imogen
dc.contributor.authorMoffett, Ashley
dc.contributor.authorBest, Serena
dc.contributor.authorTurco, Margherita Y
dc.contributor.authorBurton, Graham J
dc.contributor.authorCameron, Ruth E
dc.date.accessioned2019-11-15T00:30:38Z
dc.date.available2019-11-15T00:30:38Z
dc.date.issued2020-04-06
dc.identifier.issn2042-8898
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/298898
dc.description.abstractThe endometrium is the secretory lining of the uterus that undergoes dynamic changes throughout the menstrual cycle in preparation for implantation and a pregnancy. Recently, endometrial organoids (EO) were established to study the glandular epithelium. We have built upon this advance and developed a multi-cellular model containing both endometrial stromal and epithelial cells. We use porous collagen scaffolds produced with controlled lyophilization to direct cellular organization, integrating organoids with primary isolates of stromal cells. The internal pore structure of the scaffold was optimized for stromal cell culture in a systematic study, finding an optimal average pore size of 101 µm. EO seeded organize to form a luminal-like epithelial layer, on the surface of the scaffold. The cells polarize with their apical surface carrying microvilli and cilia that face the pore cavities and their basal surface attaching to the scaffold with the formation of extracellular matrix proteins. Both cell types are hormone responsive on the scaffold, with hormone stimulation resulting in epithelial differentiation and stromal decidualization.
dc.description.sponsorshipfunding This work was supported by the Centre for Trophoblast Research and the Wellcome Trust (090108/Z/09/Z, 085992/Z/08/Z); Y.A was supported by an Isaac Newton grant awarded to M.Y.T; L.G.B. was funded by a Marshall Scholarship from the Marshall Aid Commemoration Commission; M.Y.T. is supported by a Royal Society Dorothy Hodgkin Fellowship; S.M.B. and R.E.C. acknowledge funding from EPSRC Established Career Fellowship Grant No. EP/N019938/1.
dc.format.mediumPrint-Electronic
dc.languageeng
dc.publisherThe Royal Society
dc.rightsAll rights reserved
dc.titleGeneration of a three-dimensional collagen scaffold-based model of the human endometrium.
dc.typeArticle
prism.issueIdentifier2
prism.publicationDate2020
prism.publicationNameInterface Focus
prism.startingPage20190079
prism.volume10
dc.identifier.doi10.17863/CAM.45955
dcterms.dateAccepted2019-11-13
rioxxterms.versionofrecord10.1098/rsfs.2019.0079
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2020-04
dc.contributor.orcidAbbas, Yassen [0000-0001-8052-6691]
dc.contributor.orcidBrunel, Lucia G [0000-0003-0327-5635]
dc.identifier.eissn2042-8901
rioxxterms.typeJournal Article/Review
pubs.funder-project-idRoyal Society (DH160216)
pubs.funder-project-idWellcome Trust (085992/Z/08/Z)
pubs.funder-project-idWellcome Trust (090108/Z/09/Z)
pubs.funder-project-idEngineering and Physical Sciences Research Council (EP/N019938/1)
cam.issuedOnline2020-02-14
cam.orpheus.successTue Mar 31 10:38:49 BST 2020 - Embargo updated
cam.orpheus.counter2
rioxxterms.freetoread.startdate2020-04-30


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