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dc.contributor.authorSalasc, Fanny
dc.contributor.authorGludish, David W
dc.contributor.authorJarvis, Isobel
dc.contributor.authorBoliar, Saikat
dc.contributor.authorWills, Mark
dc.contributor.authorRussell, David G
dc.contributor.authorLever, Andrew
dc.contributor.authorMok, Hoi-Ping
dc.date.accessioned2019-11-22T00:31:03Z
dc.date.available2019-11-22T00:31:03Z
dc.date.issued2019-12-18
dc.identifier.issn2045-2322
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/299145
dc.description.abstractUnderstanding the mechanisms involved in HIV infection and latency, and development of a cure, rely on the availability of sensitive research tools such as indicator cells, which allow rigorous quantification of viral activity. Here we describe the construction and validation of a novel dual-indicator cell line, Sup-GGR, which offers two different readouts to quantify viral replication. A construct expressing both Gaussia luciferase and hrGFP in a Tat- and Rev-dependent manner was engineered into SupT1-CCR5 to create Sup-GGR cells. This cell line supports the replication of both X4 and R5-tropic HIV as efficiently as its parental cell line, SupT1-CCR5, and allows repeated sampling without the need to terminate the culture. Sup-GGR demonstrates comparable sensitivity and similar kinetics in virus outgrowth assays (VOA) to SupT1-CCR5 using clinical samples. However the Gaussia luciferase reporter is significantly less labor-intensive and allows earlier detection of reactivated latent viruses compared to the conventional HIV p24 ELISA assay. The Sup-GGR cell line constitutes a versatile new tool for HIV research and clinical trials.
dc.description.sponsorshipNational Institutes for Health Research, UK (NIHR, Cambridge Biomedical Research Centres), the Cambridge Clinical Academic Reserve and the Medical Research Council, UK (MR/N02043X/1) . This work was also supported by the National Institute of Health (AI118582 and AI36097).
dc.format.mediumElectronic
dc.languageeng
dc.publisherSpringer Science and Business Media LLC
dc.rightsAll rights reserved
dc.subjectCD4-Positive T-Lymphocytes
dc.subjectCell Line
dc.subjectHumans
dc.subjectHIV-1
dc.subjectHIV Infections
dc.subjectLuciferases
dc.subjectDisease Reservoirs
dc.subjectVirus Latency
dc.subjectVirus Replication
dc.titleA novel, sensitive dual-indicator cell line for detection and quantification of inducible, replication-competent latent HIV-1 from reservoir cells.
dc.typeArticle
prism.issueIdentifier1
prism.publicationDate2019
prism.publicationNameSci Rep
prism.startingPage19325
prism.volume9
dc.identifier.doi10.17863/CAM.46209
dcterms.dateAccepted2019-11-15
rioxxterms.versionofrecord10.1038/s41598-019-55596-8
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2019-12-18
dc.contributor.orcidSalasc, Fanny [0000-0002-6574-1358]
dc.contributor.orcidWills, Mark [0000-0001-8548-5729]
dc.contributor.orcidRussell, David G [0000-0002-9748-750X]
dc.identifier.eissn2045-2322
rioxxterms.typeJournal Article/Review
pubs.funder-project-idMedical Research Council (MR/S009752/1)
pubs.funder-project-idMedical Research Council (MR/N02043X/1)
pubs.funder-project-idMedical Research Council (MR/K021087/1)
cam.issuedOnline2019-12-18
cam.orpheus.successMon Jun 08 08:21:21 BST 2020 - The item has an open VoR version.
rioxxterms.freetoread.startdate2022-11-21


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