RORγt+ Treg to Th17 ratios correlate with susceptibility to Giardia infection.
Authors
Cortés, Alba
Klotz, Christian
Kühl, Anja A
Heimesaat, Markus M
Hartmann, Susanne
Rausch, Sebastian
Publication Date
2019-12-30Journal Title
Sci Rep
ISSN
2045-2322
Publisher
Springer Science and Business Media LLC
Volume
9
Issue
1
Pages
20328
Language
eng
Type
Article
This Version
AM
Physical Medium
Electronic
Metadata
Show full item recordCitation
Yordanova, I. A., Cortés, A., Klotz, C., Kühl, A. A., Heimesaat, M. M., Cantacessi, C., Hartmann, S., & et al. (2019). RORγt+ Treg to Th17 ratios correlate with susceptibility to Giardia infection.. Sci Rep, 9 (1), 20328. https://doi.org/10.1038/s41598-019-56416-9
Abstract
Infections with Giardia are among the most common causes of food and water-borne diarrheal disease worldwide. Here, we investigated Th17, Treg and IgA responses, and alterations in gut microbiota in two mouse lines with varying susceptibility to Giardia muris infection. Infected BALB/c mice shed significantly more cysts compared with C57BL/6 mice. Impaired control of infection in BALB/c mice was associated with lower Th17 activity and lower IgA levels compared with C57BL/6 mice. The limited metabolic activity, proliferation and cytokine production of Th17 cells in BALB/c mice was associated with higher proportions of intestinal Foxp3+RORγt+ regulatory T cells and BALB/c mice developed increased RORγt+ Treg:Th17 ratios in response to G. muris infection. Furthermore, G. muris colonization led to a significantly reduced evenness in the gut microbial communities of BALB/c mice. Our data indicate that differential susceptibility to Giardia infections may be related to RORγt+ Treg controlling Th17 activity and that changes in the microbiota composition upon Giardia infection partially depend on the host background.
Keywords
Animals, Mice, Giardia, Giardiasis, Disease Susceptibility, Genetic Predisposition to Disease, Immunoglobulin A, Lymphocyte Count, Immunophenotyping, Female, T-Lymphocytes, Regulatory, Host-Parasite Interactions, Th17 Cells, Biomarkers, Gastrointestinal Microbiome, Genetic Background
Embargo Lift Date
2022-11-26
Identifiers
External DOI: https://doi.org/10.1038/s41598-019-56416-9
This record's URL: https://www.repository.cam.ac.uk/handle/1810/299268
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