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Primary Human Testicular Cells Self-Organize into Organoids with Testicular Properties.

Published version
Peer-reviewed

Type

Article

Change log

Authors

Baert, Yoni 
Alves-Lopes, João P  ORCID logo  https://orcid.org/0000-0003-3042-6988
Söder, Olle 
Stukenborg, Jan-Bernd  ORCID logo  https://orcid.org/0000-0002-2839-1870

Abstract

So far, successful de novo formation of testicular tissue followed by complete spermatogenesis in vitro has been achieved only in rodents. Our findings reveal that primary human testicular cells are able to self-organize into human testicular organoids (TOs), i.e., multi-cellular tissue surrogates, either with or without support of a biological scaffold. Despite lacking testis-specific topography, these mini-tissues harbored spermatogonia and their important niche cells, which retained specific functionalities during long-term culture. These observations indicate the posibility of in vitro re-engineering of a human testicular microenvironment from primary cells. Human TOs might help in the development of a biomimetic testicular model that would exert a tremendous impact on research and development, clinical treatment of infertility, and screening in connection with drug discovery and toxicology.

Description

Keywords

immunofluorescence, inhibin B, in vitro spermatogenesis, organoid, scaffold, secretome, spermatogonia, testis, testosterone, whole mount, Biomarkers, Cell Culture Techniques, Cells, Cultured, Cytokines, Gene Expression, Hormones, Humans, Male, Organogenesis, Organoids, Spermatogenesis, Stem Cell Niche, Testis, Tissue Scaffolds

Journal Title

Stem Cell Reports

Conference Name

Journal ISSN

2213-6711
2213-6711

Volume Title

8

Publisher

Elsevier BV