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dc.contributor.authorFukushima, Tsuyoshi
dc.contributor.authorTanaka, Yosuke
dc.contributor.authorHamey, Fiona K
dc.contributor.authorChang, Chih-Hsiang
dc.contributor.authorOki, Toshihiko
dc.contributor.authorAsada, Shuhei
dc.contributor.authorHayashi, Yasutaka
dc.contributor.authorFujino, Takeshi
dc.contributor.authorYonezawa, Taishi
dc.contributor.authorTakeda, Reina
dc.contributor.authorKawabata, Kimihito Cojin
dc.contributor.authorFukuyama, Tomofusa
dc.contributor.authorUmemoto, Terumasa
dc.contributor.authorTakubo, Keiyo
dc.contributor.authorTakizawa, Hitoshi
dc.contributor.authorGoyama, Susumu
dc.contributor.authorIshihama, Yasushi
dc.contributor.authorHonda, Hiroaki
dc.contributor.authorGöttgens, Berthold
dc.contributor.authorKitamura, Toshio
dc.date.accessioned2019-12-17T00:30:47Z
dc.date.available2019-12-17T00:30:47Z
dc.date.issued2019-12-17
dc.identifier.issn2211-1247
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/299982
dc.description.abstractQuiescent hematopoietic stem cells (HSCs) are typically dormant, and only a few quiescent HSCs are active. The relationship between "dormant" and "active" HSCs remains unresolved. Here we generate a G0 marker (G0M) mouse line that visualizes quiescent cells and identify a small population of active HSCs (G0Mlow), which are distinct from dormant HSCs (G0Mhigh), within the conventional quiescent HSC fraction. Single-cell RNA-seq analyses show that the gene expression profiles of these populations are nearly identical but differ in their Cdk4/6 activity. Furthermore, high-throughput small-molecule screening reveals that high concentrations of cytoplasmic calcium ([Ca2+]c) are linked to dormancy of HSCs. These findings indicate that G0M separates dormant and active adult HSCs, which are regulated by Cdk4/6 and [Ca2+]c. This G0M mouse line represents a useful resource for investigating physiologically important stem cell subpopulations.
dc.format.mediumPrint
dc.languageeng
dc.publisherElsevier BV
dc.rightsAll rights reserved
dc.subjectHematopoietic Stem Cells
dc.subjectCytoplasm
dc.subjectAnimals
dc.subjectMice, Inbred C57BL
dc.subjectMice
dc.subjectCalcium
dc.subjectGene Expression Profiling
dc.subjectCell Proliferation
dc.subjectGene Expression Regulation
dc.subjectFemale
dc.subjectMale
dc.subjectGene Regulatory Networks
dc.subjectSingle-Cell Analysis
dc.subjectBiomarkers
dc.subjectCell Self Renewal
dc.subjectResting Phase, Cell Cycle
dc.titleDiscrimination of Dormant and Active Hematopoietic Stem Cells by G0 Marker Reveals Dormancy Regulation by Cytoplasmic Calcium.
dc.typeArticle
prism.endingPage4158.e7
prism.issueIdentifier12
prism.publicationDate2019
prism.publicationNameCell Rep
prism.startingPage4144
prism.volume29
dc.identifier.doi10.17863/CAM.47054
dcterms.dateAccepted2019-11-14
rioxxterms.versionofrecord10.1016/j.celrep.2019.11.061
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2019-12
dc.contributor.orcidGottgens, Berthold [0000-0001-6302-5705]
dc.identifier.eissn2211-1247
rioxxterms.typeJournal Article/Review
pubs.funder-project-idWellcome Trust (203151/Z/16/Z)
pubs.funder-project-idWellcome Trust (079895/Z/06/Z)
pubs.funder-project-idMedical Research Council (MC_PC_12009)
cam.orpheus.successTue Feb 01 18:59:04 GMT 2022 - Embargo updated
rioxxterms.freetoread.startdate2019-12-17


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