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dc.contributor.authorFukushima, Tsuyoshien
dc.contributor.authorTanaka, Yosukeen
dc.contributor.authorHamey, Fiona Ken
dc.contributor.authorChang, Chih-Hsiangen
dc.contributor.authorOki, Toshihikoen
dc.contributor.authorAsada, Shuheien
dc.contributor.authorHayashi, Yasutakaen
dc.contributor.authorFujino, Takeshien
dc.contributor.authorYonezawa, Taishien
dc.contributor.authorTakeda, Reinaen
dc.contributor.authorKawabata, Kimihito Cojinen
dc.contributor.authorFukuyama, Tomofusaen
dc.contributor.authorUmemoto, Terumasaen
dc.contributor.authorTakubo, Keiyoen
dc.contributor.authorTakizawa, Hitoshien
dc.contributor.authorGoyama, Susumuen
dc.contributor.authorIshihama, Yasushien
dc.contributor.authorHonda, Hiroakien
dc.contributor.authorGottgens, Bertholden
dc.contributor.authorKitamura, Toshioen
dc.date.accessioned2019-12-17T00:30:47Z
dc.date.available2019-12-17T00:30:47Z
dc.date.issued2019-12en
dc.identifier.issn2211-1247
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/299982
dc.description.abstractQuiescent hematopoietic stem cells (HSCs) are typically dormant, and only a few quiescent HSCs are active. The relationship between “dormant” and “active” HSCs remains unresolved. Here we generated a G0 marker (G0M) mouse line that visualizes quiescent cells. G0M identifies a small population of active HSCs (G0Mlow), which are distinct from dormant HSCs (G0Mhigh), within the conventional quiescent HSC fraction. Single-cell RNA-Seq analyses showed that the gene expression profiles of these populations were nearly identical, yet differed in their Cdk4/6 activity. Furthermore high-throughput small molecule screening revealed that high concentrations of cytoplasmic calcium ([Ca2+]c) were linked to dormancy of HSCs. These findings indicate that G0M separates dormant and active adult HSCs, which are regulated by Cdk4/6 and [Ca2+]c. This G0M mouse line represents a useful resource for investigating physiologically important stem cell subpopulations.
dc.format.mediumPrinten
dc.languageengen
dc.publisherElsevier
dc.rightsAll rights reserved
dc.rights.uri
dc.subjectHematopoietic Stem Cellsen
dc.subjectCytoplasmen
dc.subjectAnimalsen
dc.subjectMice, Inbred C57BLen
dc.subjectMiceen
dc.subjectCalciumen
dc.subjectGene Expression Profilingen
dc.subjectCell Proliferationen
dc.subjectGene Expression Regulationen
dc.subjectFemaleen
dc.subjectMaleen
dc.subjectGene Regulatory Networksen
dc.subjectSingle-Cell Analysisen
dc.subjectBiomarkersen
dc.subjectCell Self Renewalen
dc.subjectResting Phase, Cell Cycleen
dc.titleDiscrimination of Dormant and Active Hematopoietic Stem Cells by G<sub>0</sub> Marker Reveals Dormancy Regulation by Cytoplasmic Calcium.en
dc.typeArticle
prism.endingPage4158.e7
prism.issueIdentifier12en
prism.publicationDate2019en
prism.publicationNameCell reportsen
prism.startingPage4144
prism.volume29en
dc.identifier.doi10.17863/CAM.47054
dcterms.dateAccepted2019-11-14en
rioxxterms.versionofrecord10.1016/j.celrep.2019.11.061en
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2019-12en
dc.contributor.orcidHamey, Fiona K [0000-0001-7299-2860]
dc.contributor.orcidAsada, Shuhei [0000-0001-6116-7996]
dc.contributor.orcidFukuyama, Tomofusa [0000-0002-0709-3188]
dc.contributor.orcidTakubo, Keiyo [0000-0002-1736-7592]
dc.contributor.orcidTakizawa, Hitoshi [0000-0002-5276-5430]
dc.contributor.orcidGottgens, Berthold [0000-0001-6302-5705]
dc.identifier.eissn2211-1247
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idWellcome Trust (203151/Z/16/Z)
pubs.funder-project-idWellcome Trust (079895/Z/06/Z)
pubs.funder-project-idMRC (MC_PC_12009)
rioxxterms.freetoread.startdate2022-12-16


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