Comment on "Protein assemblies ejected directly from native membranes yield complexes for mass spectrometry".
Accepted version
Peer-reviewed
Repository URI
Repository DOI
Change log
Authors
Hirst, Judy https://orcid.org/0000-0001-8667-6797
Kunji, Edmund RS https://orcid.org/0000-0002-0610-4500
Walker, John E https://orcid.org/0000-0001-7929-2162
Abstract
Chorev et al (Reports, 16 November 2018, p. 829) describe mass spectrometry on mitochondrial membrane proteins ionized directly from their native environment. However, the assignments made to measured masses are incorrect or inconclusive and lack experimental validation. The proteins are not in their "native" condition: They have been stripped of tightly bound lipids, and the complexes are fragmented or in physiologically irrelevant oligomeric states.
Description
Keywords
Lipids, Mass Spectrometry, Membrane Proteins
Journal Title
Science
Conference Name
Journal ISSN
0036-8075
1095-9203
1095-9203
Volume Title
366
Publisher
American Association for the Advancement of Science (AAAS)
Publisher DOI
Rights
All rights reserved
Sponsorship
Medical Research Council (MC_UU_00015/1)
Medical Research Council (MC_EX_MR/M009858/1)
Medical Research Council (MC_U105663139)
MRC (MC_UP_1002/1)
Medical Research Council (MC_U105663141)
Medical Research Council (MC_U105663150)
Medical Research Council (MC_UU_00015/8)
MRC (MC_UU_00015/2)
MRC (MC_UU_00015/8)
Medical Research Council (MC_UP_1002/1)
Medical Research Council (MC_UU_00015/7)
Medical Research Council (MC_EX_MR/M009858/1)
Medical Research Council (MC_U105663139)
MRC (MC_UP_1002/1)
Medical Research Council (MC_U105663141)
Medical Research Council (MC_U105663150)
Medical Research Council (MC_UU_00015/8)
MRC (MC_UU_00015/2)
MRC (MC_UU_00015/8)
Medical Research Council (MC_UP_1002/1)
Medical Research Council (MC_UU_00015/7)