Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons.
Minett, Michael S
Flanagan, John G
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Koppers, M., Cagnetta, R., Shigeoka, T., Wunderlich, L. C., Vallejo-Ramirez, P., Qiaojin Lin, J., Zhao, S., et al. (2019). Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons.. https://doi.org/10.7554/eLife.48718
Extrinsic cues trigger the local translation of specific mRNAs in growing axons via cell surface receptors. The coupling of ribosomes to receptors has been proposed as a mechanism linking signals to local translation but it is not known how broadly this mechanism operates, nor whether it can selectively regulate mRNA translation. We report that receptor-ribosome coupling is employed by multiple guidance cue receptors and this interaction is mRNA-dependent. We find that different receptors associate with distinct sets of mRNAs and RNA-binding proteins. Cue stimulation of growing Xenopus retinal ganglion cell axons induces rapid dissociation of ribosomes from receptors and the selective translation of receptor-specific mRNAs. Further, we show that receptor-ribosome dissociation and cue-induced selective translation are inhibited by co-exposure to translation-repressive cues, suggesting a novel mode of signal integration. Our findings reveal receptor-specific interactomes and suggest a generalizable model for cue-selective control of the local proteome.
Human, mRNA, RNA-binding protein, Xenopus, Neuroscience, Retinal ganglion cell, Axon, developmental biology, Local Protein Synthesis, Guidance Receptor
Netherlands Organisation for Scientific Research (Rubicon 019.161LW.033)
Netherlands Organization for Scientific Research (Rubicon 019.161LW.033)
European Research Council (Advanced Grant 322817, 322817)
Wellcome Trust (203249/Z/16/Z, 089703/Z/09/Z, 3-3249/Z/16/Z, 085314/Z/08/Z)
EPSRC (EP/L015889/1, EP/H018301/1)
External DOI: https://doi.org/10.7554/eLife.48718
This record's URL: https://www.repository.cam.ac.uk/handle/1810/300241
Attribution 4.0 International
Licence URL: https://creativecommons.org/licenses/by/4.0/