Show simple item record

dc.contributor.authorSelley, Lizaen
dc.contributor.authorSchuster, Lindaen
dc.contributor.authorMarbach, Heleneen
dc.contributor.authorForsthuber, Theresaen
dc.contributor.authorForbes, Benen
dc.contributor.authorGant, Timothy Wen
dc.contributor.authorSandström, Thomasen
dc.contributor.authorCamiña, Nuriaen
dc.contributor.authorAthersuch, Toby Jen
dc.contributor.authorMudway, Ianen
dc.contributor.authorKumar, Abhinaven
dc.date.accessioned2020-01-03T09:26:30Z
dc.date.available2020-01-03T09:26:30Z
dc.date.issued2020-03en
dc.identifier.issn1756-5901
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/300366
dc.description.abstractStudies have emphasised the importance of combustion-derived particles in eliciting adverse health effects, especially those produced by diesel vehicles. In contrast, few investigations have explored the potential toxicity of particles derived from tyre and brake wear, despite their significant contributions to total roadside particulate mass. The objective of this study was to compare the relative toxicity of compositionally distinct brake abrasion dust (BAD) and diesel exhaust particles (DEP) in a cellular model that is relevant to human airways. Although BAD contained considerably more metals/ metalloids than DEP (as determined by inductively coupled plasma mass spectrometry) similar toxicological profiles were observed in U937 monocyte-derived macrophages following 24h exposures to 4-25 µg/ml doses of either particle type. Responses to the particles were characterised by dose-dependent decreases in mitochondrial depolarisation (p ≤ 0.001), increased secretion of IL-8, IL-10 and TNF-α (p ≤ 0.05- p ≤ 0.001) and decreased phagocytosis of S.aureus (p ≤ 0.001). This phagocytic deficit recovered, and the inflammatory response resolved when challenged cells were incubated for a further 24h in particle-free media. These responses were abrogated by metal chelation using desferroxamine. At minimally cytotoxic doses both DEP and BAD perturbed bacterial clearance and promoted inflammatory responses in U937 cell with similar potency. These data emphasise the requirement to consider contributions of abrasion particles to traffic-related clinical health effects.
dc.description.sponsorshipMRC
dc.format.mediumPrinten
dc.languageengen
dc.publisherRSC
dc.rightsAll rights reserved
dc.rights.uri
dc.titleBrake dust exposure exacerbates inflammation and transiently compromises phagocytosis in macrophages.en
dc.typeArticle
prism.endingPage386
prism.issueIdentifier3en
prism.publicationDate2020en
prism.publicationNameMetallomics : integrated biometal scienceen
prism.startingPage371
prism.volume12en
dc.identifier.doi10.17863/CAM.47440
rioxxterms.versionofrecord10.1039/c9mt00253gen
rioxxterms.versionAM*
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2020-03en
dc.contributor.orcidSelley, Liza [0000-0002-0651-2790]
dc.contributor.orcidForbes, Ben [0000-0001-8193-6107]
dc.contributor.orcidGant, Timothy W [0000-0001-9057-4937]
dc.contributor.orcidCamiña, Nuria [0000-0002-0714-7705]
dc.contributor.orcidAthersuch, Toby J [0000-0002-5732-9574]
dc.contributor.orcidMudway, Ian [0000-0003-1239-5014]
dc.identifier.eissn1756-591X
rioxxterms.typeJournal Article/Reviewen
cam.orpheus.successThu Jan 30 10:34:37 GMT 2020 - Embargo updated*
rioxxterms.freetoread.startdate2021-01-09


Files in this item

Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record