Criticality of plasma membrane lipids reflects activation state of macrophage cells.
Veatch, Sarah L
Journal of the Royal Society, Interface
The Royal Society
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Cammarota, E., Soriani, C., Taub, R., Morgan, F., Sakai, J., Veatch, S. L., Bryant, C., & et al. (2020). Criticality of plasma membrane lipids reflects activation state of macrophage cells.. Journal of the Royal Society, Interface, 17 (163), 20190803. https://doi.org/10.1098/rsif.2019.0803
Signalling is of particular importance in immune cells, and upstream in the signalling pathway many membrane receptors are functional only as complexes, co-locating with particular lipid species. Work over the last 15 years has shown that plasma membrane lipid composition is close to a critical point of phase separation, with evidence that cells adapt their composition in ways that alter the proximity to this thermodynamical point. Macrophage cells are a key component of the innate immune system, responsive to infections, regulating the local state of inflammation. We investigate changes in the plasma membrane’s proximity to the critical point, as a response to stimulation by various pro- and anti-inflammatory agents. Pro-inflammatory (IFN- γ , Kdo-LipidA, LPS) perturbations induce an increase in the transition temperature of the GMPVs; anti-inflammatory IL4 has the opposite effect. These changes recapitulate complex plasma membrane composition changes, and are consistent with lipid criticality playing a master regulatory role: being closer to critical conditions increases membrane protein activity.
Research was funded by EUMarie Curie action ITN TransPol (EC), NIH-R01GM110052 and NSF10 MCB1552439 (SLV), Cambridge University Commonwealth, European and International Trust 11 (JS) ITN BioPol (PC), and Wellcome Trust Investigator grant 08045/Z/15/Z (CEB).
WELLCOME TRUST (108045/Z/15/Z)
External DOI: https://doi.org/10.1098/rsif.2019.0803
This record's URL: https://www.repository.cam.ac.uk/handle/1810/301750
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