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dc.contributor.authorDoria Ramirez, Maria Gabriela
dc.date.accessioned2020-02-07T14:30:07Z
dc.date.available2020-02-07T14:30:07Z
dc.date.issued2020-02-22
dc.date.submitted2019-04-16
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/301823
dc.description.abstractPetal epidermal cell shape has been shown to affect pollination success in flowering plants. Conical epidermal cells may increase grip for insect pollinators and enhance flower colouration compared to non-conical cells. Nicotiana (Solanaceae) presents a diverse range of petal cell shapes. Interestingly, sister species in at least two phylogenetically distinct clades of the genus have contrasting petal epidermal cell shapes (conical vs. non-conical). This project aims to further understand character evolution of petal cell shape in Nicotiana and its implications in pollination systems, combining tools of molecular biology, morphology and pollinator behaviour experiments. First, using a candidate gene approach, I explore in parallel the molecular mechanisms involved in petal cell shape differentiation of sister species with contrasting cell shape N. cordifolia and N. solanifolia (Section Paniculatae) and N. bonariensis and N. forgetiana (Section Alatae). Subgroup 9 R2R3 MYB transcription factors are potentially responsible for the molecular control of petal cell shape. Differential expression of subgroup 9 R2R3 MYBs in petals of the sister species, rather than sequence differences in these genes, might be explaining the contrasting cell morphologies. Next, I develop an Agrobacterium tumefaciens mediated transformation protocol for the non-model species N. forgetiana, a methodological advance crucial for further exploration of the molecular mechanisms and functional implications of petal cell shape. Lastly, I explore how petal colour and petal cell shape interact in the perception of flowers by model pollinator Bombus terrestris using biomimetic artificial flowers. Pollinator behaviour experiments indicate that the bumblebees can discriminate flowers with conical from flowers with non-conical surfaces, on a red and on a white background, using visual cues alone as well as tactile cues alone. This investigation improves our understanding of the molecular mechanisms involved in petal epidermal cell morphogenesis and of the functional implications of petal cell shape in the interaction flower-pollinator in Nicotiana.
dc.description.sponsorshipCambridge International Trust and the Newton-Caldas Fund partnership between the British Council (UK) and Colciencias (Colombia)
dc.language.isoen
dc.rightsAll rights reserved
dc.rightsAll Rights Reserveden
dc.rights.urihttps://www.rioxx.net/licenses/all-rights-reserved/en
dc.subjectAgrobacterium tumefaciens
dc.subjectangiosperms
dc.subjectartificial flowers
dc.subjectAtacama Desert
dc.subjectbee learning
dc.subjectbee pollination
dc.subjectbee vision
dc.subjectbee hexagon
dc.subjectbird pollination
dc.subjectBombus terrestris
dc.subjectbumblebees
dc.subjectconical cells
dc.subjectdifferential conditioning
dc.subjectepidermal cells
dc.subjectevo-devo
dc.subjectevolution
dc.subjectfloral features
dc.subjectflowering plants
dc.subjectflowers
dc.subjectforaging speed
dc.subjectfower development
dc.subjectfunction
dc.subjectgene expression
dc.subjectgene regulation
dc.subjectgene sequencing
dc.subjectJuan Fernandez Islands
dc.subjectMIXTA genes
dc.subjectMYB genes
dc.subjectmoth pollination
dc.subjectNicotiana
dc.subjectNicotiana benthamiana
dc.subjectNicotiana bonariensis
dc.subjectNicotiana cordifolia
dc.subjectNicotiana forgetiana
dc.subjectNicotiana solanifolia
dc.subjectNicotiana tabacum
dc.subjectpetal cell shape
dc.subjectpetal texture
dc.subjectphylogenetics
dc.subjectplant evolution
dc.subjectplant molecular biology
dc.subjectplant tissue culture
dc.subjectpollination
dc.subjectpollinator behaviour
dc.subjectpollination sysndrome
dc.subjectprotein function
dc.subjectR2R3 Subgroup 9 MYB
dc.subjectSolanaceae
dc.subjecttobacco
dc.subjecttranscription factors
dc.titlePetal cell shape and flower-pollinator interaction in Nicotiana
dc.typeThesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnameDoctor of Philosophy (PhD)
dc.publisher.institutionUniversity of Cambridge
dc.publisher.departmentDepartment of Plant Sciences
dc.date.updated2020-02-04T16:11:55Z
dc.identifier.doi10.17863/CAM.48891
dc.contributor.orcidDoria Ramirez, Maria Gabriela [0000-0002-8144-1144]
dc.publisher.collegeWolfson College
dc.type.qualificationtitlePhD in Plant Sciences
cam.supervisorGlover, Beverley
cam.thesis.fundingfalse
rioxxterms.freetoread.startdate2021-02-07


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