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dc.contributor.authorStubbs, Samuel C B
dc.contributor.authorQuaye, Osbourne
dc.contributor.authorAcquah, Maame Ekua
dc.contributor.authorAdadey, Samuel Mawuli
dc.contributor.authorKean, Iain R L
dc.contributor.authorGupta, Srishti
dc.contributor.authorBlacklaws, Barbara A
dc.date.accessioned2020-02-12T01:45:49Z
dc.date.available2020-02-12T01:45:49Z
dc.date.issued2020-01-10
dc.identifier.issn1746-6148
dc.identifier.otherPMC6954506
dc.identifier.other31924206
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/302024
dc.description.abstractBACKGROUND:The introduction of rotavirus A vaccination across the developing world has not proved to be as efficacious as first hoped. One cause of vaccine failure may be infection by zoonotic rotaviruses that are very variable antigenically from the vaccine strain. However, there is a lack of genomic information about the circulating rotavirus A strains in farm animals in the developing world that may be a source of infection for humans. We therefore screened farms close to Accra, Ghana for animals sub-clinically infected with rotavirus A and then sequenced the virus found in one of these samples. RESULTS:6.1% of clinically normal cows and pigs tested were found to be Rotavirus A virus antigen positive in the faeces. A subset of these (33.3%) were also positive for virus RNA. The most consistently positive pig sample was taken forward for metagenomic sequencing. This gave full sequence for all open reading frames except segment 5 (NSP1), which is missing a single base at the 5' end. The virus infecting this pig had genome constellation G5-P[7]-I5-R1-C1-M1-A8-N1-T7-E1-H1, a known porcine genotype constellation. CONCLUSIONS:Farm animals carry rotavirus A infection sub-clinically at low frequency. Although the rotavirus A genotype discovered here has a pig-like genome constellation, a number of the segments most closely resembled those isolated from humans in suspected cases of zoonotic transmission. Therefore, such viruses may be a source of variable gene segments for re-assortment with other viruses to cause vaccine breakdown. It is recommended that further human and pig strains are characterized in West Africa, to better understand this dynamic.
dc.languageeng
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceessn: 1746-6148
dc.sourcenlmid: 101249759
dc.subjectPig
dc.subjectPorcine
dc.subjectGhana
dc.subjectRotavirus A
dc.subjectFull Genome Sequence Constellation
dc.titleFull genomic characterization of a porcine rotavirus strain detected in an asymptomatic piglet in Accra, Ghana.
dc.typeArticle
dc.date.updated2020-02-12T01:45:48Z
prism.issueIdentifier1
prism.publicationNameBMC veterinary research
prism.volume16
dc.identifier.doi10.17863/CAM.49100
rioxxterms.versionofrecord10.1186/s12917-019-2226-9
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidQuaye, Osbourne [0000-0002-0621-876X]


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International