Transposon age and non-CG methylation.
MetadataShow full item record
Wang, Z., & Baulcombe, D. (2020). Transposon age and non-CG methylation.. Nature communications, 11 (1), 1221. https://doi.org/10.1038/s41467-020-14995-6
Silencing of transposable elements (TEs) is established by small RNA-directed DNA methylation (RdDM). Maintenance of silencing is then based on a combination of RdDM and RNA-independent mechanisms involving DNA methyltransferase MET1 and chromodomain DNA methyltransferases (CMTs). Involvement of RdDM, according to this model should decrease with TE age but here we show a different pattern in tomato and Arabidopsis. In these species the CMTs silence long terminal repeat (LTR) transposons in the distal chromatin that are younger than those affected by RdDM. To account for these findings we propose that, after establishment of primary RdDM as in the original model, there is an RNA-independent maintenance phase involving CMTs followed by secondary RdDM. This progression of epigenetic silencing in the gene-rich distal chromatin is likely to influence the transcriptome either in cis or in trans depending on whether the mechanisms are RNA-dependent or -independent.
Chromatin, Lycopersicon esculentum, RNA Polymerase II, Nucleotides, Plant Proteins, DNA Transposable Elements, Evolution, Molecular, DNA Methylation, Gene Silencing, Terminal Repeat Sequences, Phenotype, Mutation, CRISPR-Cas Systems
This work was supported by European Research Council Advanced Investigator grant ERC-2013-AdG 340642 (Transgressive Inheritance in plant Breeding and Evolution [TRIBE]), the Royal Society (RP170001), the Balzan Foundation and BBSRC grant BB/R018529/1. D.C.B. is the Royal Society Edward Penley Abraham Research Professor.
International Balzan Prize Foundation (10957)
Royal Society (RP170001)
Royal Society (RP/EA/180018)
European Research Council (340642)
Embargo Lift Date
External DOI: https://doi.org/10.1038/s41467-020-14995-6
This record's URL: https://www.repository.cam.ac.uk/handle/1810/302171
All rights reserved