Meiotic gene silencing complex MTREC/NURS recruits the nuclear exosome to YTH-RNA-binding protein Mmi1
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Publication Date
2020-02-03Journal Title
PLOS Genetics
ISSN
1553-7390
Publisher
Public Library of Science
Volume
16
Issue
2
Language
en
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Shichino, Y., Otsubo, Y., Yamamoto, M., & Yamashita, A. (2020). Meiotic gene silencing complex MTREC/NURS recruits the nuclear exosome to YTH-RNA-binding protein Mmi1. PLOS Genetics, 16 (2)https://doi.org/10.1371/journal.pgen.1008598
Abstract
Accurate target recognition in transcript degradation is crucial for regulation of gene expression. In the fission yeast Schizosaccharomyces pombe, a number of meiotic transcripts are recognized by a YTH-family RNA-binding protein, Mmi1, and selectively degraded by the nuclear exosome during mitotic growth. Mmi1 forms nuclear foci in mitotically growing cells, and the nuclear exosome colocalizes to such foci. However, it remains elusive how Mmi1 and the nuclear exosome are connected. Here, we show that a complex called MTREC (Mtl1-Red1 core) or NURS (nuclear RNA silencing) that consists of a zinc-finger protein, Red1, and an RNA helicase, Mtl1, is required for the recruitment of the nuclear exosome to Mmi1 foci. Physical interaction between Mmi1 and the nuclear exosome depends on Red1. Furthermore, a chimeric protein involving Mmi1 and Rrp6, which is a nuclear-specific component of the exosome, suppresses the ectopic expression phenotype of meiotic transcripts in red1Δ cells and mtl1 mutant cells. These data indicate that the primary function of MTREC/NURS in meiotic transcript elimination is to link Mmi1 to the nuclear exosome physically.
Keywords
Research Article, Biology and life sciences, Research and analysis methods
Identifiers
pgenetics-d-19-01079
External DOI: https://doi.org/10.1371/journal.pgen.1008598
This record's URL: https://www.repository.cam.ac.uk/handle/1810/302181
Rights
Licence:
http://creativecommons.org/licenses/by/4.0/