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dc.contributor.authorZoulias, Nicholas
dc.contributor.authorBrown, Jordan
dc.contributor.authorRowe, James
dc.contributor.authorCasson, Stuart A
dc.date.accessioned2020-02-19T11:33:12Z
dc.date.available2020-02-19T11:33:12Z
dc.date.issued2020-01-16
dc.identifier.issn1932-6203
dc.identifier.otherPMC6964886
dc.identifier.other31945058
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/302375
dc.description.abstractLight is a crucial signal that regulates many aspects of plant physiology and growth including the development of stomata, the pores in the epidermal surface of the leaf. Light signals positively regulate stomatal development leading to changes in stomatal density and stomatal index (SI; the proportion of cells in the epidermis that are stomata). Both phytochrome and cryptochrome photoreceptors are required to regulate stomatal development in response to light. The transcription factor ELONGATED HYPOCOTYL 5 (HY5) is a key regulator of light signalling, acting downstream of photoreceptors. We hypothesised that HY5 could regulate stomatal development in response to light signals due to the putative presence of HY5 binding sites in the promoter of the STOMAGEN (STOM) gene, which encodes a peptide regulator of stomatal development. Our analysis shows that HY5 does have the potential to regulate the STOM promoter in vitro and that HY5 is expressed in both the epidermis and mesophyll. However, analysis of hy5 and hy5 hyh double mutants (HYH; HY5-HOMOLOG), found that they had normal stomatal development under different light conditions and the expression of stomatal developmental genes was not perturbed following light shift experiments. Analysis of stable lines overexpressing HY5 also showed no change in stomatal development or the expression of stomatal developmental genes. We therefore conclude that whilst HY5 has the potential to regulate the expression of STOM, it does not have a major role in regulating stomatal development in response to light signals.
dc.languageeng
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceessn: 1932-6203
dc.sourcenlmid: 101285081
dc.titleHY5 is not integral to light mediated stomatal development in Arabidopsis.
dc.typeArticle
dc.date.updated2020-02-19T11:33:12Z
prism.issueIdentifier1
prism.publicationNamePloS one
prism.volume15
dc.identifier.doi10.17863/CAM.49447
rioxxterms.versionofrecord10.1371/journal.pone.0222480
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidZoulias, Nicholas [0000-0002-2505-2734]
dc.contributor.orcidRowe, James [0000-0002-3523-4347]
dc.contributor.orcidCasson, Stuart A [0000-0001-5702-6947]
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/N002393/1)


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International