High-throughput, multi-parametric, and correlative fluorescence lifetime imaging.
Publication Date
2020-02-20Journal Title
Methods Appl Fluoresc
ISSN
2050-6120
Publisher
IOP Publishing
Volume
8
Issue
2
Language
en
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Poudel, C., Mela, I., & Kaminski, C. (2020). High-throughput, multi-parametric, and correlative fluorescence lifetime imaging.. Methods Appl Fluoresc, 8 (2) https://doi.org/10.1088/2050-6120/ab7364
Description
Funder: Infinitus (China), Ltd.
Funder: MedImmune (AstraZeneca); doi: https://doi.org/10.13039/501100004628
Abstract
In this review, we discuss methods and advancements in fluorescence lifetime imaging microscopy that permit measurements to be performed at faster speed and higher resolution than previously possible. We review fast single-photon timing technologies and the use of parallelized detection schemes to enable high-throughput and high content imaging applications. We appraise different technological implementations of fluorescence lifetime imaging, primarily in the time-domain. We also review combinations of fluorescence lifetime with other imaging modalities to capture multi-dimensional and correlative information from a single sample. Throughout the review, we focus on applications in biomedical research. We conclude with a critical outlook on current challenges and future opportunities in this rapidly developing field.
Keywords
Paper, Special Issue on Fluorescence Lifetime Imaging (FLIM): from Fundamentals to Applications, fluorescence lifetime, acquisition time, SPAD array, high throughput, FLIM, multi-parametric imaging, correlative FLIM
Sponsorship
Engineering and Physical Sciences Research Council (EP/H018301/1)
Wellcome Trust (089703/Z/09/Z)
Medical Research Council (MR/K015850/1)
Medical Research Council (MR/K02292X/1)
Engineering and Physical Sciences Research Council (EP/L015889/1)
European Commission Horizon 2020 (H2020) Marie Sk?odowska-Curie actions (722380)
Biotechnology and Biological Sciences Research Council (BB/H023917/1)
Wellcome Trust (203249/Z/16/Z)
Identifiers
mafab7364, ab7364, maf-100664.r2
External DOI: https://doi.org/10.1088/2050-6120/ab7364
This record's URL: https://www.repository.cam.ac.uk/handle/1810/302438
Rights
Attribution 4.0 International (CC BY 4.0)
Licence URL: https://creativecommons.org/licenses/by/4.0/
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