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dc.contributor.authorDong, Fangen
dc.contributor.authorHao, Shaen
dc.contributor.authorZhang, Senen
dc.contributor.authorZhu, Caiyingen
dc.contributor.authorCheng, Huien
dc.contributor.authorYang, Ziningen
dc.contributor.authorHamey, Fiona Ken
dc.contributor.authorWang, Xiaofangen
dc.contributor.authorGao, Aien
dc.contributor.authorWang, Fengjiaoen
dc.contributor.authorGao, Yunen
dc.contributor.authorDong, Jien
dc.contributor.authorWang, Chenchenen
dc.contributor.authorWang, Jinyongen
dc.contributor.authorLan, Yuen
dc.contributor.authorLiu, Bingen
dc.contributor.authorEma, Hideoen
dc.contributor.authorTang, Fuchouen
dc.contributor.authorGottgens, Bertholden
dc.contributor.authorZhu, Pingen
dc.contributor.authorCheng, Taoen
dc.date.accessioned2020-03-05T00:30:46Z
dc.date.available2020-03-05T00:30:46Z
dc.date.issued2020-06en
dc.identifier.issn1465-7392
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/303053
dc.description.abstractHow transplanted haematopoietic stem cells (HSCs) reside and behave in a preconditioned host at the early stage has not been studied due to technical limitations. Here, by single-cell RNA-sequencing, we first obtained the transcriptome-based classifications of 28 haematopoietic cell types, and then applied them in conjunction with functional assays to track the dynamic changes of highly purified HSCs in irradiated recipients within the first week after transplantation. Based on our transcriptional classifications, most homed HSCs in bone marrow and spleen were shown to become multipotent progenitors and occasionally, some HSCs gave rise to megakaryocytic/erythroid or myeloid precursors. Parallel in vitro and in vivo functional experiments supported the paradigm of vigorous differentiation without substantial HSC expansion during the first week. Therefore, this study uncovers the previously inaccessible kinetics and fate choices of transplanted HSCs in myeloablated recipients at early stage, with implications for clinical applications of HSCs and other stem cells.
dc.description.sponsorshipMRC (MR/K500975/1, MC_PC_12009), Wellcome Trust (203151/Z/16/Z)
dc.format.mediumPrint-Electronicen
dc.languageengen
dc.publisherSpringer Nature
dc.rightsAll rights reserved
dc.rights.uri
dc.subjectHematopoietic Stem Cellsen
dc.subjectMegakaryocytesen
dc.subjectMyeloid Cellsen
dc.subjectAnimalsen
dc.subjectMice, Inbred C57BLen
dc.subjectMiceen
dc.subjectCell Cycleen
dc.subjectCell Differentiationen
dc.subjectCell Lineageen
dc.subjectFemaleen
dc.subjectErythroid Precursor Cellsen
dc.subjectSingle-Cell Analysisen
dc.subjectTranscriptomeen
dc.titleDifferentiation of transplanted haematopoietic stem cells tracked by single-cell transcriptomic analysis.en
dc.typeArticle
prism.endingPage639
prism.issueIdentifier6en
prism.publicationDate2020en
prism.publicationNameNature cell biologyen
prism.startingPage630
prism.volume22en
dc.identifier.doi10.17863/CAM.50129
dcterms.dateAccepted2020-03-30en
rioxxterms.versionofrecord10.1038/s41556-020-0512-1en
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2020-06en
dc.contributor.orcidZhang, Sen [0000-0003-3849-6817]
dc.contributor.orcidHamey, Fiona K [0000-0001-7299-2860]
dc.contributor.orcidGao, Yun [0000-0003-3530-7259]
dc.contributor.orcidWang, Jinyong [0000-0002-7218-0659]
dc.contributor.orcidLan, Yu [0000-0002-4731-5945]
dc.contributor.orcidTang, Fuchou [0000-0002-8625-7717]
dc.contributor.orcidGöttgens, Berthold [0000-0001-6302-5705]
dc.contributor.orcidZhu, Ping [0000-0002-4521-7524]
dc.contributor.orcidCheng, Tao [0000-0003-4395-1095]
dc.identifier.eissn1476-4679
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idWellcome Trust (203151/Z/16/Z)
pubs.funder-project-idMRC (MR/K500975/1)
pubs.funder-project-idMRC (MC_PC_12009)
cam.orpheus.successMon Jun 01 08:40:34 BST 2020 - Embargo updated*
cam.orpheus.counter8*
rioxxterms.freetoread.startdate2020-11-04


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