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Genome-wide CRISPR screening identifies new regulators of glycoprotein secretion.

Published version
Peer-reviewed

Type

Article

Change log

Authors

Villeneuve, Julien 
Perez Garcia, Esther 
Petrunkina Harrison, Anna 

Abstract

Background: The fundamental process of protein secretion from eukaryotic cells has been well described for many years, yet gaps in our understanding of how this process is regulated remain. Methods: With the aim of identifying novel genes involved in the secretion of glycoproteins, we used a screening pipeline consisting of a pooled genome-wide CRISPR screen, followed by secondary siRNA screening of the hits to identify and validate several novel regulators of protein secretion. Results: We present approximately 50 novel genes not previously associated with protein secretion, many of which also had an effect on the structure of the Golgi apparatus. We further studied a small selection of hits to investigate their subcellular localisation. One of these, GPR161, is a novel Golgi-resident protein that we propose maintains Golgi structure via an interaction with golgin A5. Conclusions: This study has identified new factors for protein secretion involved in Golgi homeostasis.

Description

Keywords

CRISPR screen, Golgi, endoplasmic reticulum, secretion

Journal Title

Wellcome Open Res

Conference Name

Journal ISSN

2398-502X
2398-502X

Volume Title

4

Publisher

F1000 Research Ltd
Sponsorship
Wellcome Trust (100574/Z/12/Z)
Medical Research Council (MC_UU_12012/5)
Wellcome Trust (109152/Z/15/Z)
Biotechnology and Biological Sciences Research Council (BB/P010911/1)
Medical Research Council (MC_PC_12012)