Genome-wide CRISPR screening identifies new regulators of glycoprotein secretion.
Perez Garcia, Esther
Petrunkina Harrison, Anna
Wellcome open research
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Popa, S., Villeneuve, J., Stewart, S., Perez Garcia, E., Petrunkina Harrison, A., & Moreau, K. (2019). Genome-wide CRISPR screening identifies new regulators of glycoprotein secretion.. Wellcome open research, 4 https://doi.org/10.12688/wellcomeopenres.15232.2
Background: The fundamental process of protein secretion from eukaryotic cells has been well described for many years, yet gaps in our understanding of how this process is regulated remain. Methods: With the aim of identifying novel genes involved in the secretion of glycoproteins, we used a screening pipeline consisting of a pooled genome-wide CRISPR screen, followed by secondary siRNA screening of the hits to identify and validate several novel regulators of protein secretion. Results: We present approximately 50 novel genes not previously associated with protein secretion, many of which also had an effect on the structure of the Golgi apparatus. We further studied a small selection of hits to investigate their subcellular localisation. One of these, GPR161, is a novel Golgi-resident protein that we propose maintains Golgi structure via an interaction with golgin A5. Conclusions: This study has identified new factors for protein secretion involved in Golgi homeostasis.
Secretion, Endoplasmic reticulum, Golgi, Crispr Screen
Medical Research Council (MC_UU_12012/5)
Wellcome Trust (100574, 109152)
External DOI: https://doi.org/10.12688/wellcomeopenres.15232.2
This record's URL: https://www.repository.cam.ac.uk/handle/1810/303254
Attribution 4.0 International
Licence URL: https://creativecommons.org/licenses/by/4.0/