Organic phase separation opens up new opportunities to interrogate the RNA-binding proteome.
Published version
Peer-reviewed
Repository URI
Repository DOI
Change log
Authors
Smith, Tom https://orcid.org/0000-0002-0697-8777
Villanueva, Eneko
Queiroz, Rayner ML
Dawson, Charlotte S
Elzek, Mohamed
Abstract
Protein-RNA interactions regulate all aspects of RNA metabolism and are crucial to the function of catalytic ribonucleoproteins. Until recently, the available technologies to capture RNA-bound proteins have been biased toward poly(A) RNA-binding proteins (RBPs) or involve molecular labeling, limiting their application. With the advent of organic-aqueous phase separation-based methods, we now have technologies that efficiently enrich the complete suite of RBPs and enable quantification of RBP dynamics. These flexible approaches to study RBPs and their bound RNA open up new research avenues for systems-level interrogation of protein-RNA interactions.
Description
Keywords
Mass spectrometry, Organic phase separation, RNA-binding proteins, RNA-sequencing, Transcriptome, Proteome, Proteomics, RNA, RNA, Messenger, RNA-Binding Proteins
Journal Title
Curr Opin Chem Biol
Conference Name
Journal ISSN
1367-5931
1879-0402
1879-0402
Volume Title
54
Publisher
Elsevier BV
Publisher DOI
Sponsorship
Wellcome Trust (110071/Z/15/Z)
Wellcome Trust (110170/Z/15/Z)
Biotechnology and Biological Sciences Research Council (BB/N010493/1)
Wellcome Trust (110170/Z/15/Z)
Biotechnology and Biological Sciences Research Council (BB/N010493/1)