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dc.contributor.authorHuefner, Annaen
dc.contributor.authorKuan, Williamen
dc.contributor.authorBarker, Rogeren
dc.contributor.authorMahajan, Sumeeten
dc.date.accessioned2020-03-21T00:30:09Z
dc.date.available2020-03-21T00:30:09Z
dc.date.issued2013-06en
dc.identifier.issn1530-6984
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/303651
dc.description.abstractDistinction between closely related and morphologically similar cells is difficult by conventional methods especially without labeling. Using nuclear-targeted gold nanoparticles (AuNPs) as intracellular probes we demonstrate the ability to distinguish between progenitor and differentiated cell types in a human neuroblastoma cell line using surface-enhanced Raman spectroscopy (SERS). SERS spectra from the whole cell area as well as only the nucleus were analyzed using principal component analysis that allowed unambiguous distinction of the different cell types. SERS spectra from the nuclear region showed the developments during cellular differentiation by identifying an increase in DNA/RNA ratio and proteins transcribed. Our approach using nuclear-targeted AuNPs and SERS imaging provides label-free and noninvasive characterization that can play a vital role in identifying cell types in biomedical stem cell research.
dc.format.mediumPrint-Electronicen
dc.languageengen
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectNeuronsen
dc.subjectCell Line, Tumoren
dc.subjectHumansen
dc.subjectProteinsen
dc.subjectDNAen
dc.subjectRNAen
dc.subjectMolecular Probesen
dc.subjectSpectrum Analysis, Ramanen
dc.subjectNanotechnologyen
dc.titleIntracellular SERS nanoprobes for distinction of different neuronal cell types.en
dc.typeArticle
prism.endingPage2470
prism.issueIdentifier6en
prism.publicationDate2013en
prism.publicationNameNano lettersen
prism.startingPage2463
prism.volume13en
dc.identifier.doi10.17863/CAM.50728
rioxxterms.versionofrecord10.1021/nl400448nen
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2013-06en
dc.contributor.orcidKuan, Wei-Li [0000-0002-0273-1320]
dc.contributor.orcidBarker, Roger [0000-0001-8843-7730]
dc.contributor.orcidMahajan, Sumeet [0000-0001-8923-6666]
dc.identifier.eissn1530-6992
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idEPSRC (EP/H028757/1)
pubs.funder-project-idEPSRC (EP/G060649/1)
pubs.funder-project-idEPSRC (EP/K028510/1)
pubs.funder-project-idWellcome Trust (079249/Z/06/H)


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International